Molaparast Morteza, Eslampour Pooya, Soleymani Jafar, Shafiei-Irannejad Vahid
Cellular and Molecular Research Center, Cellular and Molecular Medicine Institute, Urmia University of Medical Sciences, Urmia, Iran.
Food and Drug Safety Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
Iran J Pharm Res. 2022 May 12;21(1):e126918. doi: 10.5812/ijpr-126918. eCollection 2022 Dec.
For the first time, nitrogen, sulfur, phosphorus, and boron-doped carbon dots (N, S, P, B-codoped CDs) were synthesized through a hydrothermal reaction. The produced CDs were utilized to develop an optical sensor to determine methotrexate (MTX) in cell lysates and patients' plasma samples. Basically, in the presence of MTX, the fluorescence emission of the CD-based probe was quenched. Under optimum conditions, a good proportional relationship was obtained between the quenched fluorescence signal and MTX concentrations from 74.9 ng/mL to 99.9 µg/mL with a limit of detection of 74.9 ng/mL. The developed nanoprobe provided a wide linear range and high accuracy and was successfully utilized in the routine therapeutic drug monitoring of MTX in plasma samples. The obtained results proposed the developed nanoprobe for the on-time and specific detection of MTX in blood samples. As another application, N, S, P, B-codoped CDs were utilized for bioimaging MCF-7 cancer cells and could be proposed as efficient bioimaging agents for tumor cells.
首次通过水热反应合成了氮、硫、磷和硼共掺杂的碳点（N、S、P、B共掺杂碳点）。所制备的碳点被用于开发一种光学传感器，以测定细胞裂解液和患者血浆样本中的甲氨蝶呤（MTX）。基本上，在MTX存在的情况下，基于碳点的探针的荧光发射会被淬灭。在最佳条件下，淬灭的荧光信号与MTX浓度在74.9 ng/mL至99.9 μg/mL之间呈现良好的比例关系，检测限为74.9 ng/mL。所开发的纳米探针具有宽线性范围和高准确度，并成功用于血浆样本中MTX的常规治疗药物监测。所得结果表明所开发的纳米探针可用于及时、特异性地检测血液样本中的MTX。作为另一个应用，N、S、P、B共掺杂碳点被用于MCF-7癌细胞的生物成像，并可被提议作为肿瘤细胞的高效生物成像剂。
