Asadzadeh Aghdaei Hamid, Rezasoltani Sama, Olfatifar Meisam, Nazemalhosseini Mojarad Ehsan, Sherkat Ghazal, Yadegar Abbas, Feizabadi Mohammad Mehdi, Zali Mohammad Reza
Basic and Molecular Epidemiology of Gastrointestinal Disorders Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Foodborne and Waterborne Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Avicenna J Med Biotechnol. 2022 Jul-Sep;14(3):188-195. doi: 10.18502/ajmb.v14i3.9825.
Toll-Like Receptors (TLRs) are the critical mediators of inflammatory routs in the gut, which play an essential role in regulating the immune responses towards various ligands derived from pathogenic bacteria. Also, TLR signaling has been implicated in the development of Inflammatory Bowel Disease (IBD), Adenomatous Polyp (AP), and Colorectal Cancer (CRC). Here, we aimed to examine the expression of some TLRs concerning certain fecal bacteria in AP and CRC patients with and without IBD.
This case-control study collected fecal and colonic tissue samples from 93 patients versus Normal Controls (NC) colonoscopy. Fecal samples were used for DNA extraction, and the abundance of selected fecal bacteria was determined by absolute real-time PCR. Also, the gene expression of TLR2, 4, and 5 was analyzed using RT-PCR on the colonic tissues of participants.
Compared to NC individuals, in AP and CRC patients, the mRNA expressions of TLR4 and TLR2 were significantly increased while TLR5 was decreased. A meaningful association between TLRs mRNA expression levels and the abundance of some selected fecal bacteria was detected. Also, there was a significant relationship between participant's food regimes, smoking habit and intestinal TLRs expression.
Our study proposed the important role of TLRs during adenomatous and CRC formation. Alterations in TLRs expression associated with certain gut bacteria may contribute to disease development.
Toll样受体（TLRs）是肠道炎症途径的关键介质，在调节对源自病原菌的各种配体的免疫反应中起重要作用。此外，TLR信号传导与炎症性肠病（IBD）、腺瘤性息肉（AP）和结直肠癌（CRC）的发生有关。在此，我们旨在研究在有或没有IBD的AP和CRC患者中，某些TLRs与特定粪便细菌的表达情况。
本病例对照研究通过结肠镜检查从93例患者与正常对照（NC）中收集粪便和结肠组织样本。粪便样本用于DNA提取，通过绝对实时PCR测定所选粪便细菌的丰度。此外，使用RT-PCR分析参与者结肠组织中TLR2、4和5的基因表达。
与NC个体相比，AP和CRC患者中TLR4和TLR2的mRNA表达显著增加，而TLR5表达降低。检测到TLRs mRNA表达水平与一些所选粪便细菌丰度之间存在有意义的关联。此外，参与者的饮食习惯、吸烟习惯与肠道TLRs表达之间存在显著关系。
我们的研究提出了TLRs在腺瘤性息肉和CRC形成过程中的重要作用。与某些肠道细菌相关的TLRs表达改变可能有助于疾病发展。
