Rezasoltani Sama, Khatibi Shirin, Pezeshkiyan Zahra, Nazemalhosseini-Mojarad Ehsan, Sharafkhah Maryam, Sadeghi Amir, Asadzadeh Aghdaei Hamid, Zali Mohammad Reza
Basic and Molecular Epidemiology of Gastrointestinal Disorders Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Gastroenterology and Liver Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran. Email:
Asian Pac J Cancer Prev. 2019 Aug 1;20(8):2299-2302. doi: 10.31557/APJCP.2019.20.8.2299.
Toll-like receptor 9 (TLR9) is a cellular DNA receptor of the innate immune system which plays a pivotal role in inflammatory response. Recently, changing expression levels of TLR9 has been observed in a wide range of cancer cells; however, there is little information about colorectal polyps. Herein, we assessed the mRNA expression of TLR9 in different colorectal polyp types compared to normal group in order to investigate its expression level during CRC initiation. Fifty-four biopsy samples from colorectal polyp patients and from 20 healthy subjects were collected. The mucosal mRNA expression level of TLR9 gene was identified by real time PCR. Fold change of gene expression was evaluated by 2-ΔΔct method. There was a significant relationship between the lower expression of TLR9 gene in the polyp cases compared to normal individuals (P value = 0.0005), Also, decreased TLR9 mRNA expression was obtained in adenomas in contrast to hyperplastic and normal groups (P value = 0.0008). Based on the current results, we hypothesized that aberrant surface expression of TLR9 on tumor cells may promote the growth and invasion of colorectal polyps. Further, TLR9 modulation may have an important impact on the development of novel therapeutic strategies.
Toll样受体9（TLR9）是天然免疫系统的一种细胞DNA受体，在炎症反应中起关键作用。最近，在多种癌细胞中观察到TLR9表达水平的变化；然而，关于大肠息肉的信息却很少。在此，我们评估了与正常组相比不同类型大肠息肉中TLR9的mRNA表达，以研究其在结直肠癌起始过程中的表达水平。收集了54例大肠息肉患者和20名健康受试者的活检样本。通过实时PCR鉴定TLR9基因的黏膜mRNA表达水平。采用2-ΔΔct法评估基因表达的倍数变化。与正常个体相比，息肉病例中TLR9基因表达较低存在显著相关性（P值=0.0005），此外，与增生性和正常组相比，腺瘤中TLR9 mRNA表达降低（P值=0.0008）。基于目前的结果，我们推测肿瘤细胞上TLR9的异常表面表达可能促进大肠息肉的生长和侵袭。此外，TLR9调节可能对新型治疗策略的开发具有重要影响。
