Waterborg J H, Winicov I, Harrington R E
Arch Biochem Biophys. 1987 Jul;256(1):167-78. doi: 10.1016/0003-9861(87)90435-8.
The histones from the alfalfa plant Medicago sativa have been characterized in terms of type variants and levels of acetylation. Histones were isolated directly from total plant tissue (callus), eliminating the need to develop methods for nuclear isolation. An acid-urea-polyacrylamide gel with a transverse Triton X-100 gradient resolved and identified in a single gel at least one type of histone H4, two variant forms of histone H2B, two variant forms of histone H3, and four variant forms of histone H2A from a crude histone preparation. Histone H4 was present 25% in an unmodified state and 75% as monomodified, presumably as monoacetylated histone. Both histone H3 variants displayed five bands, consistent with up to four internal sites of acetylation. The two H3 variants differed in their steady-state level of acetylation, suggesting that they may reside in different chromatin environments. Several histone H1 species were identified by solubility and cross-reactivity with antiserum raised against the globular part of bovine H1(0), indicating conservation of epitopes between histone H1 of mammals and higher plants.
紫花苜蓿（Medicago sativa）植株的组蛋白已根据类型变体和乙酰化水平进行了表征。组蛋白直接从植物总组织（愈伤组织）中分离出来，无需开发细胞核分离方法。一种带有横向 Triton X - 100梯度的酸性尿素聚丙烯酰胺凝胶在单一凝胶中解析并鉴定了来自粗制组蛋白制剂的至少一种H4组蛋白类型、两种H2B组蛋白变体形式、两种H3组蛋白变体形式以及四种H2A组蛋白变体形式。H4组蛋白25%以未修饰状态存在，75%为单修饰状态，推测为单乙酰化组蛋白。两种H3组蛋白变体均显示五条带，这与多达四个内部乙酰化位点一致。这两种H3变体在其乙酰化稳态水平上有所不同，表明它们可能存在于不同的染色质环境中。通过溶解性以及与针对牛H1(0)球状部分产生的抗血清的交叉反应性鉴定出了几种H1组蛋白，这表明哺乳动物和高等植物的H1组蛋白之间存在表位保守性。
