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小麦、大麦、水稻和玉米中组蛋白H3变体的多样性。

Multiplicity of histone h3 variants in wheat, barley, rice, and maize.

作者信息

Waterborg J H

机构信息

Division of Cell Biology and Biophysics, School of Basic Life Sciences, University of Missouri-Kansas City, Kansas City, Missouri 64110-2499.

出版信息

Plant Physiol. 1991 Jun;96(2):453-8. doi: 10.1104/pp.96.2.453.

DOI:10.1104/pp.96.2.453
PMID:16668207
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1080791/
Abstract

Histone H3 proteins were purified to near homogeneity from etiolated seedlings of wheat (Triticum aestivum), barley (Hordeum vulgare), rice (Oryza sativa), maize (Zea mays), and alfalfa (Medicago sativa) to determine the number of histone H3 variants. Five distinct histone H3 variants were identified by gradient gel electrophoresis and reversed phase chromatography. These variants occur in various combinations of two to four forms in each plant species. One minor histone variant form (variant III, named H3.2 in alfalfa) appeared present and identical in all mono- and dicotyledonous plant species tested to date. All H3 proteins were acetylated to multiple levels and in every species the variant III form was acetylated most extensively. The level of histone H3 acetylation showed an inverse correlation with plant genome size. These observations support the idea that acetylated histones and especially variant III proteins are an element of transcriptionally active chromatin.

摘要

从小麦(Triticum aestivum)、大麦(Hordeum vulgare)、水稻(Oryza sativa)、玉米(Zea mays)和苜蓿(Medicago sativa)的黄化幼苗中纯化组蛋白H3至接近均一,以确定组蛋白H3变体的数量。通过梯度凝胶电泳和反相色谱法鉴定出五种不同的组蛋白H3变体。这些变体在每个植物物种中以两种至四种形式的各种组合出现。一种次要的组蛋白变体形式(变体III,在苜蓿中命名为H3.2)在迄今为止测试的所有单子叶和双子叶植物物种中似乎都存在且相同。所有H3蛋白都被乙酰化到多个水平,并且在每个物种中,变体III形式的乙酰化程度最高。组蛋白H3乙酰化水平与植物基因组大小呈负相关。这些观察结果支持这样的观点,即乙酰化组蛋白,尤其是变体III蛋白是转录活性染色质的一个组成部分。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f443/1080791/77ac2eb33955/plntphys00693-0115-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f443/1080791/c894c6709724/plntphys00693-0113-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f443/1080791/16d4ddfd4a7c/plntphys00693-0114-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f443/1080791/77ac2eb33955/plntphys00693-0115-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f443/1080791/c894c6709724/plntphys00693-0113-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f443/1080791/16d4ddfd4a7c/plntphys00693-0114-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f443/1080791/77ac2eb33955/plntphys00693-0115-a.jpg

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Multiplicity of histone h3 variants in wheat, barley, rice, and maize.小麦、大麦、水稻和玉米中组蛋白H3变体的多样性。
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Fractionation and characterization of histones from barley (Hordeum vulgare) leaves : Existence of multiple H2A and H2B variants.从大麦(Hordeum vulgare)叶片中分离和鉴定组蛋白:存在多种 H2A 和 H2B 变体。
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Dynamic methylation of alfalfa histone H3.
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本文引用的文献

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Phosphorylation of Plant H2A Histones.植物 H2A 组蛋白的磷酸化。
Plant Physiol. 1990 Jul;93(3):1241-5. doi: 10.1104/pp.93.3.1241.
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Differential Histone Acetylation in Alfalfa (Medicago sativa) Due to Growth in NaCl : Responses in Salt Stressed and Salt Tolerant Callus Cultures.苜蓿(紫花苜蓿)中因NaCl生长导致的组蛋白乙酰化差异:盐胁迫和耐盐愈伤组织培养物中的响应
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Histone Hyperacetylation in Maize in Response to Treatment with HC-Toxin or Infection by the Filamentous Fungus Cochliobolus carbonum.玉米中组蛋白的高度乙酰化对HC毒素处理或丝状真菌玉米炭疽菌感染的响应
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