Wright H T, Robertus J D
Arch Biochem Biophys. 1987 Jul;256(1):280-4. doi: 10.1016/0003-9861(87)90447-4.
Alkylation of the cysteine residues which link the A and B chains of ricin through a disulfide bridge produces a molecule which still binds to HeLa cells and is toxic toward in vitro ribosome-directed translation, but which has little or no cytotoxicity toward cells in culture. This and similar observations on diphtheria toxin implicate the intersubunit disulfide bridge in the transport of the toxic subunits of these toxins into the cytoplasm.
通过二硫键连接蓖麻毒素A链和B链的半胱氨酸残基发生烷基化反应后，会产生一种分子，该分子仍能与HeLa细胞结合，并且对体外核糖体指导的翻译具有毒性，但对培养中的细胞几乎没有细胞毒性。对白喉毒素的这一观察结果以及类似观察结果表明，这些毒素的毒性亚基向细胞质的转运与亚基间的二硫键有关。
