Sakamoto H, Yoshida T, Miyagawa K, Terada M
Gan To Kagaku Ryoho. 1987 Jun;14(6 Pt 2):2147-51.
We have identified a novel transforming gene, hst, from a human stomach cancer, and the results of our studies on hst are reviewed. From a total of 26 patients with stomach cancer, DNAs from 21 of their stomach cancers, 16 metastatic lymph node tumors from stomach cancers and 21 non-cancerous stomach mucosae, were assayed for their transforming activity to NIH3T3 cells. Three samples of DNA were positive; one was from a primary stomach cancer, the second from a non-cancerous portion of the same patient's stomach mucosa, and the third from a lymph node metastasis stomach cancer from a different patient. A portion of the transforming gene and near full-length cDNA for this gene was cloned. From the results of cDNA sequence and Southern blot analyses, this transforming gene was shown to have no homology with the oncogene reported previously. We applied the term, hst (human stomach cancer), to this novel transforming gene. The hst gene was found to be responsible for acquisition of transforming activity of all the three different samples of DNAs.
我们从一例人类胃癌中鉴定出一种新的转化基因hst，并对我们关于hst的研究结果进行综述。在总共26例胃癌患者中，检测了来自其中21例患者的胃癌DNA、16例胃癌转移淋巴结肿瘤DNA以及21例非癌性胃黏膜DNA对NIH3T3细胞的转化活性。有3份DNA样本呈阳性；一份来自原发性胃癌，第二份来自同一患者胃黏膜的非癌部分，第三份来自另一位患者的胃癌淋巴结转移灶。克隆了该转化基因的部分片段和近全长cDNA。根据cDNA序列和Southern印迹分析结果，该转化基因与先前报道的癌基因无同源性。我们将这个新的转化基因命名为hst（人类胃癌）。发现hst基因是所有这3份不同DNA样本获得转化活性的原因。
