Yoshida T, Sakamoto H, Miyagawa K, Odagiri H, Terada M
Genetics division, National Cancer Center Research Institute.
Hum Cell. 1988 Mar;1(1):10-6.
The hst gene was originally identified in surgically obtained human gastric mucosae as a transforming gene which could transform NIH3T3 cells morphologically. The hst cDNA clone was synthesized from mRNA of one of the NIH3T3 transformants. A human leukocyte genomic library was screened with this cDNA clone, and an hst genomic fragment was obtained. This genomic fragment itself had transforming activity, and the protein coding sequences were proved to be completely identical to those of the cDNA clone prepared from mRNA of the NIH3T3 transformant. This fact suggests that rearrangement or other structural alterations in the coding sequence are not required for the activation of the hst gene. The predicted hst protein consists of 206 amino acids and has a significant homology (40-50%) to fibroblast growth factors and int-2 protein. They together make up a new superfamily of growth factors and transforming genes.
hst基因最初是在手术获取的人胃黏膜中作为一种能在形态上转化NIH3T3细胞的转化基因而被鉴定出来的。hst cDNA克隆是从其中一个NIH3T3转化体的mRNA合成的。用该cDNA克隆筛选人白细胞基因组文库，获得了一个hst基因组片段。这个基因组片段本身具有转化活性，并且蛋白质编码序列被证明与从NIH3T3转化体的mRNA制备的cDNA克隆的编码序列完全相同。这一事实表明，hst基因的激活不需要编码序列的重排或其他结构改变。预测的hst蛋白由206个氨基酸组成，与成纤维细胞生长因子和int-2蛋白有显著的同源性（40-50%）。它们共同构成了一个新的生长因子和转化基因超家族。
