Ahmad Zishan, Yadav Vikas, Shahzad Anwar, Emamverdian Abolghassem, Ramakrishnan Muthusamy, Ding Yulong
Co-Innovation Centre for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing, China.
Bamboo Research Institute, Nanjing Forestry University, Nanjing, China.
Front Plant Sci. 2022 Aug 18;13:905444. doi: 10.3389/fpls.2022.905444. eCollection 2022.
is an important tree of the forest, cultivated in tropical and subtropical regions, providing fuelwood, land reclamation, dune stabilization, paper production, and nitrogen fixation. We have developed a systematic propagation protocol in using nodal segments (NS). Murashige and Skoog (MS) medium augmented with BA (5.0 μM) and NAA (0.5 μM) gave rise to a maximum of 32.00 ± 0.31 shoots per explant (S/E) with shoot length (SL) of 3.94 ± 0.02 cm, and a maximum of 70% regeneration potential (RP) was recorded after 8 weeks of post inoculation. For root induction, derived shoots were transferred to the nutrient medium consisting of a half-strength (½) MS medium augmented with 2.5 μM NAA, which produced a maximum of 12.68 ± 0.33 roots/shoot (R/S) with 3.04 ± 0.50 cm root length (RL) in 60% of culture after 6 weeks. Micropropagated plants with healthy shoots and roots were successfully acclimatized in vermicompost + garden soil + sand (1:2:1) and a maximum survival percentage of 95.1% was recorded. NS was taken from a 6-weeks-old derived plant of for synthetic seed production, and it was reported that CaCl · 2HO (100 mM) + Na-alginate (4%) resulted in clear and uniform beads. Furthermore, the maximum conversion of synthetic seeds into plantlets occurred over a period of 4 weeks of storage at 4°C. Scanning Electron Microscopy (SEM) revealed the formation of direct shoot buds without any intermediate callus formation. In addition, the chlorophyll and carotenoid contents of the direct regenerated and mother plant were compared. Similarly, RAPD and ISSR primers were used for genetic homogeneity assessment of the direct regenerated plants, where a total of 18 and 19, respectively, clear and reproducible bands with 100% monomorphism were recorded. The developed micropropagation protocol can certainly be used for large-scale multiplication and germplasm preservation of . It will also help in meeting the growing demands of in the forest industry.
是森林中的一种重要树木，在热带和亚热带地区种植，可提供薪材、土地开垦、沙丘固定、纸张生产以及固氮。我们已经开发了一种使用节段（NS）进行系统繁殖的方案。添加了BA（5.0 μM）和NAA（0.5 μM）的Murashige和Skoog（MS）培养基，每个外植体（S/E）最多可产生32.00 ± 0.31个芽，芽长（SL）为3.94 ± 0.02厘米，接种8周后记录到最大再生潜力（RP）为70%。为了诱导生根，将诱导出的芽转移到由添加了2.5 μM NAA的半强度（½）MS培养基组成的营养培养基中，6周后，60%的培养物中每个芽（R/S）最多可产生12.68 ± 0.33条根，根长（RL）为3.04 ± 0.50厘米。具有健康芽和根的微繁殖植株成功地在蚯蚓堆肥+园土+沙子（1:2:1）中驯化，记录到的最大存活百分比为95.1%。从6周龄的诱导植株上获取节段用于合成种子生产，据报道CaCl₂·2H₂O（100 mM）+海藻酸钠（4%）可产生清晰且均匀的珠子。此外，合成种子在4°C下储存4周的时间内，转化为植株的比例最高。扫描电子显微镜（SEM）显示直接形成了芽，没有任何中间愈伤组织的形成。此外，还比较了直接再生植株和母本植株的叶绿素和类胡萝卜素含量。同样，使用RAPD和ISSR引物对直接再生植株进行遗传同质性评估，分别记录到总共18条和19条清晰且可重复的条带，单态性为100%。所开发的微繁殖方案肯定可用于大规模繁殖和种质保存。它也将有助于满足森林工业中对其不断增长的需求。
