Alan Aydın, Alan Emel, Arslan Korhan, Daldaban Fadime, Aksel Esma Gamze, Çınar Mehmet Ulaş, Akyüz Bilal
Department of Anatomy, Faculty of Veterinary Medicine, Erciyes University, 38030 Kayseri, Turkey.
Department of Histology and Embryology, Faculty of Veterinary Medicine, Erciyes University, 38030 Kayseri, Turkey.
Microsc Microanal. 2022 Sep 5:1-15. doi: 10.1017/S1431927622012314.
Toll-like receptor (TLR)-mediated inflammatory processes play a critical role in the innate immune response during the initial interaction between the infecting microorganism and immune cells. This study aimed to investigate the possible microanatomical and histological differences in mandibular and bronchial lymph nodes in Akkaraman and Romanov lambs induced by lipopolysaccharide (LPS) and lipoteichoic acid (LTA) and study the gene, protein, and immunoexpression levels of , () and tumor necrosis factor-α () that are involved in the immune system. Microanatomical examinations demonstrated more intense lymphocyte infiltration in the bronchial lymph nodes of Akkaraman lambs in the LPS and LTA groups compared to Romanov lambs. TLR4, MyD88, and TNF-α immunoreactivities were more intense in the experimental groups of both breeds. Expression levels of and genes in the bronchial lymph node of Akkaraman lambs were found to increase statistically significantly in the LTA group. gene expression level in the mandibular lymph node was found to be statistically significantly higher in the LTA + LPS group. In conclusion, dynamic changes in the immune cell populations involved in response to antigens such as LTA and LPS in the lymph nodes of both breeds can be associated with the difference in the expression level of the genes.
Toll样受体（TLR）介导的炎症过程在感染微生物与免疫细胞初次相互作用期间的固有免疫反应中起关键作用。本研究旨在调查脂多糖（LPS）和脂磷壁酸（LTA）诱导的阿卡拉曼羊和罗曼诺夫羊下颌淋巴结和支气管淋巴结可能存在的微观解剖学和组织学差异，并研究参与免疫系统的、（）和肿瘤坏死因子-α（）的基因、蛋白质及免疫表达水平。微观解剖学检查表明，与罗曼诺夫羊相比，LPS和LTA组阿卡拉曼羊支气管淋巴结中的淋巴细胞浸润更强烈。两个品种的实验组中TLR4、MyD88和TNF-α免疫反应性更强。发现LTA组阿卡拉曼羊支气管淋巴结中基因和的表达水平有统计学显著升高。发现LTA + LPS组下颌淋巴结中基因表达水平有统计学显著升高。总之，两个品种羊淋巴结中参与对LTA和LPS等抗原反应的免疫细胞群体的动态变化可能与基因表达水平的差异有关。
