Research Institute of Clinical and Experimental Lymphology - Affiliated Branch of Federal Research Center Institute of Cytology and Genetics, Siberian Division of the Russian Academy of Sciences, Novosibirsk, Russia.
Scientific Center for Family Health and Human Reproduction Problems, Irkutsk, Russia.
Bull Exp Biol Med. 2022 Aug;173(4):569-574. doi: 10.1007/s10517-022-05590-4. Epub 2022 Sep 5.
We performed an immunohistochemical study of MT melatonin receptor expression in the liver of C57BL/6 mice with modeled light-induced functional pinealectomy and after melatonin administration by the indirect avidin-biotin peroxidase ABC method. The animals were kept for 14 days under constant lighting. Intragastric administration of melatonin in physiological doses (1 mg/kg body weight for 14 days) to mice with light-induced functional pinealectomy resulted in a 2-fold increase in the relative expression area of MT receptors in liver cells in comparison with that in animals kept under standard lighting conditions, 24-h lighting for 14 days, or 24-h lighting receiving placebo (intragastric administration of 200 ml distilled water). Melatonin treatment had practically no effect on MT staining intensity. Our results attest to the important role of MT receptors in melatonin synthesis disorders and can serve as the basis for the development of therapeutic strategies aimed at melatonin receptors.
我们通过间接亲和素-生物素过氧化物酶 ABC 法，对光照诱导的 C57BL/6 小鼠功能性松果腺切除术和褪黑素给药后的肝脏 MT 褪黑素受体表达进行了免疫组织化学研究。这些动物在持续光照下饲养了 14 天。对光照诱导的功能性松果腺切除术小鼠给予生理剂量的褪黑素（1mg/kg 体重，14 天），与在标准光照条件下饲养的动物（24 小时光照，14 天）或接受安慰剂（200ml 蒸馏水灌胃）的动物相比，肝实质细胞中 MT 受体的相对表达面积增加了 2 倍。褪黑素治疗对 MT 染色强度几乎没有影响。我们的研究结果证明了 MT 受体在褪黑素合成紊乱中的重要作用，并为旨在开发针对褪黑素受体的治疗策略提供了依据。
