PG and Research Department of Chemistry, Thiagarajar College, Madurai, Tamil Nadu, India.
Luminescence. 2022 Nov;37(11):1991-2001. doi: 10.1002/bio.4381. Epub 2022 Sep 19.
Highly fluorescent and water-soluble carbon quantum dots (CQDs) were synthesized from the bio-waste source of garlic peels (renovation of bio-waste into bio-asset) using a controlled carbonization method. Synthesized CQDs were characterized by various analytical methods and explored as a fluorogenic probe for the recognition of quercetin (QT). Ultraviolet-visible (UV-vis) result shows an absorption maximum at 275 nm attributed to the conjugation of C=C and C=O of CQDs and demonstrates a blue emission in the range 330-410 nm. Selectivity was performed with various biomolecules, except for QT, all others do not exhibit any considerable change in the fluorescence of CQDs. On the interaction with QT, emission was completely quenched due to Förster energy transfer (FET), confirming the high selective to QT. Effect of pH, sensitivity, and stability studies displayed excellent results under optimized conditions. The limit of detection (LOD) fluorescent probe was found to be 6.73 μM. Our approach may suggest a new platform for the development of quick and low-cost CQDs-based sensors for environmental and biological purposes.
采用碳化法，以大蒜皮（生物废弃物转化为生物资产）为生物废物来源，合成了高荧光性和水溶性的碳量子点（CQDs）。合成的 CQDs 通过各种分析方法进行了表征，并作为用于识别槲皮素（QT）的荧光探针进行了探索。紫外可见（UV-vis）结果显示，在 275nm 处有一个吸收最大值，归因于 CQDs 的 C=C 和 C=O 的共轭，并在 330-410nm 范围内显示出蓝色发射。除了 QT 之外，与各种生物分子进行了选择性研究，所有其他分子的 CQDs 荧光均未发生任何明显变化。与 QT 相互作用时，由于Förster 能量转移（FET），发射完全被猝灭，证实了对 QT 的高选择性。在优化条件下，对 pH 值、灵敏度和稳定性的研究显示出了优异的结果。荧光探针的检出限（LOD）被发现为 6.73μM。我们的方法可能为用于环境和生物目的的快速且低成本的基于 CQDs 的传感器的开发提供了一个新平台。
