一种与 SNW/SKI-INTERACTING PROTEIN 相互作用的蛋白影响拟南芥的内复制和细胞生长。
An SNW/SKI-INTERACTING PROTEIN influences endoreduplication and cell growth in Arabidopsis.
机构信息
State Key Laboratory of Plant Cell and Chromosome Engineering, CAS Centre for Excellence in Molecular Plant Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.
State Key Laboratory of Crop Stress Adaptation and Improvement, Collaborative Innovation Center of Crop Stress Biology, College of Life Sciences, Henan University, Kaifeng, Henan 475001, China.
出版信息
Plant Physiol. 2022 Nov 28;190(4):2217-2228. doi: 10.1093/plphys/kiac415.
Endoreduplication plays an important role in cell growth and differentiation, but the mechanisms regulating endoreduplication are still elusive. We have previously reported that UBIQUITIN-SPECIFIC PROTEASE14 (UBP14) encoded by DA3 interacts with ULTRAVIOLETB INSENSITIVE4 (UVI4) to influence endoreduplication and cell growth in Arabidopsis (Arabidopsis thaliana). The da3-1 mutant possesses larger cotyledons and flowers with higher ploidy levels than the wild-type. Here, we identify the suppressor of da3-1 (SUPPRESSOR OF da3-1 3; SUD3), which encodes SNW/SKI-INTERACTING PROTEIN (SKIP). Biochemical studies demonstrate that SUD3 physically interacts with UBP14/DA3 and UVI4 in vivo and in vitro. Genetic analyses support that SUD3 acts in a common pathway with UBP14/DA3 and UVI4 to control endoreduplication. Our findings reveal an important genetic and molecular mechanism by which SKIP/SUD3 associates with UBP14/DA3 and UVI4 to modulate endoreduplication.
内复制在细胞生长和分化中起着重要作用，但调节内复制的机制仍难以捉摸。我们之前曾报道过，由 DA3 编码的泛素特异性蛋白酶 14（UBP14）与 ULTRAVIOLETB INSENSITIVE4（UVI4）相互作用，影响拟南芥（Arabidopsis thaliana）的内复制和细胞生长。da3-1 突变体的子叶和花比野生型更大，具有更高的倍性水平。在这里，我们鉴定了 da3-1 的抑制子（SUPPRESSOR OF da3-1 3；SUD3），它编码 SNW/SKI-INTERACTING PROTEIN（SKIP）。生化研究表明，SUD3 在体内和体外与 UBP14/DA3 和 UVI4 物理相互作用。遗传分析支持 SUD3 与 UBP14/DA3 和 UVI4 共同作用，控制内复制。我们的研究结果揭示了一个重要的遗传和分子机制，即 SKIP/SUD3 与 UBP14/DA3 和 UVI4 结合，调节内复制。
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