SKIP 通过拟南芥 SEF pre-mRNA 的选择性剪接来控制开花时间。

BACKGROUND

Similar to other eukaryotes, splicing is emerging as an important process affecting development and stress tolerance in plants. Ski-interacting protein (SKIP), a splicing factor, is essential for circadian clock function and abiotic stress tolerance; however, the mechanisms whereby it regulates flowering time are unknown.

RESULTS

In this study, we found that SKIP is required for the splicing of serrated leaves and early flowering (SEF) pre-messenger RNA (mRNA), which encodes a component of the ATP-dependent SWR1 chromatin remodeling complex (SWR1-C). Defects in the splicing of SEF pre-mRNA reduced H2A.Z enrichment at FLC, MAF4, and MAF5, suppressed the expression of these genes, and produced an early flowering phenotype in skip-1 plants.

CONCLUSIONS

Our findings indicate that SKIP regulates SWR1-C function via alternative splicing to control the floral transition in Arabidopsis thaliana.

背景

与其他真核生物类似，剪接作为一个影响植物发育和抗逆性的重要过程正在兴起。剪接因子 Ski-interacting protein（SKIP）对于生物钟功能和非生物胁迫耐受性是必需的；然而，其调节开花时间的机制尚不清楚。

结果

在这项研究中，我们发现 SKIP 对于锯齿状叶片和早期开花（SEF）前信使 RNA（mRNA）的剪接是必需的，SEF 编码 ATP 依赖性 SWR1 染色质重塑复合物（SWR1-C）的一个组成部分。SEF 前 mRNA 剪接缺陷会降低 FLC、MAF4 和 MAF5 处的 H2A.Z 富集，抑制这些基因的表达，并在 skip-1 植物中产生早花表型。