Reproductive Department, Wuhan KangJian Maternity and Infant Hospital, Wuhan, Hubei, China.
Acta Biochim Pol. 2022 Sep 5;69(3):599-604. doi: 10.18388/abp.2020_5951.
MicroRNAs (miR) have been shown to exhibit marked dysregulation in polycystic ovarian syndrome (PCOS). Additionally, granulosa cell proliferation and apoptosis are important in PCOS pathogenesis. Considering this, the current study aimed at characterization of the regulatory function of miR-197-3p on the growth and proliferation of ovarian granulosa cells at the molecular level. The results showed that miR-197-3p is significantly repressed (P<0.05) in PCOS tissues and granulosa cells. Granulosa cell proliferation was significantly (P<0.05) inhibited by miR-197-3p overexpression via induction of apoptosis. In silico analysis predicted cullin 3 (CUL3) as the regulatory target of miR-197-3p, which was also confirmed by the dual luciferase reporter assay. CUL3 was overexpressed in KGN ovarian granulosa cells, and its silencing mimicked the tumor suppressive effects of miR-197-3p. Taken together, miR-197-3p exhibits a negative regulatory role in regulating granulosa ovarian cell proliferation via posttranscriptional repression of CUL3 in PCOS.
微小 RNA（miR）在多囊卵巢综合征（PCOS）中表现出明显的失调。此外，颗粒细胞的增殖和凋亡在 PCOS 的发病机制中很重要。鉴于此，本研究旨在从分子水平上描述 miR-197-3p 对卵巢颗粒细胞生长和增殖的调节功能。结果表明，miR-197-3p 在 PCOS 组织和颗粒细胞中显著受到抑制（P<0.05）。通过诱导细胞凋亡，miR-197-3p 的过表达显著抑制（P<0.05）颗粒细胞的增殖。计算机分析预测 cullin 3（CUL3）是 miR-197-3p 的调节靶点，双荧光素酶报告基因实验也证实了这一点。CUL3 在 KGN 卵巢颗粒细胞中过表达，其沉默模拟了 miR-197-3p 的肿瘤抑制作用。综上所述，miR-197-3p 通过对 PCOS 中 CUL3 的转录后抑制，对调节颗粒细胞卵巢细胞增殖发挥负调节作用。
