比较苯甲酸雌二醇和 17β-雌二醇加孕酮对定时人工授精方案中卵泡更替和发育以及妊娠结局的影响。
Comparing the effect of estradiol benzoate and 17β-estradiol plus progesterone on follicular turnover and development, and pregnancy outcomes in a timed artificial insemination protocol.
机构信息
Department of Animal Reproduction, School of Veterinary Medicine and Animal Science, University of São Paulo, Pirassununga, São Paulo, Brazil; Department of Animal Science, University of Florida, Gainesville, Florida, USA.
Department of Animal Science, University of Florida, Gainesville, Florida, USA; Department of Animal and Dairy Sciences and Brown Loam Experiment Station, Mississippi State University, Mississippi State, Mississippi, USA.
出版信息
Theriogenology. 2022 Oct 15;192:73-80. doi: 10.1016/j.theriogenology.2022.08.033. Epub 2022 Aug 28.
The objective of this study was to compare the efficiency of estradiol benzoate (EB) and 17β-estradiol (E2) associated with progesterone (P4) in a fixed-time artificial insemination (FTAI) protocol. We hypothesized that E2+P4 induces an earlier emergence of a new follicular wave (NFW), improving pre-ovulatory follicle diameter and pregnancy rates to FTAI (P/FTAI). In Exp.1, on Day 0 (D0), all Bos indicus cows (n = 12/group) received an intravaginal P4 device and a dose of PGF2α analogue. On D0, females were randomly assigned to receive EB or E2+P4. On D8.5, P4 intravaginal devices were removed and a dose of PGF2α and EB were administered in all females followed by fixed-timed AI on D10. Between D0 and D10, the dominant follicular growth was determined by ovary ultrasonography exams. On D8.5 and D10 the percentage of color power-Doppler signals in the dominant follicular wall was evaluated. In Exp. 2, 467 females (2-year-old nulliparous [n = 76], primiparous [n = 92] and pluriparous [n = 299]) were subjected to the similar FTAI and assigned to be treated with EB (n = 243) or E2+P4 (n = 224). Pregnancy diagnosis was performed 30 days after FTAI by ultrasonography. The day to emergence of NFW was similar between treatments (EB: 3.7 ± 0.37 vs. E2+P4: 3.3 ± 0.3, P = 0.76). Females treated with E2+P4 presented greater (P = 0.06) follicular growth between the emergence and D9 (1.18 ± 0.07) than those treated with EB (0.97 ± 0.08). There was also a positive effect (P < 0.05) of E2+P4 on diameter of the dominant follicle on D9 (13.0 ± 0.6 vs. 10.9 ± 0.55) and blood perfusion of the follicle wall on D8.5 (49 vs. 40%). There was a treatment by parity category interaction effect on P/FTAI (P < 0.05). Treatment with E2+P4 was advantageous to P/FTAI of primiparous cows (E2+P4: 58% and EB: 30%). However, for nulliparous and pluriparous cows, P/FTAI was similar between treatments (∼50%). In conclusion, in a E2/P4-based protocol for FTAI, E2+P4 is as efficient as EB in inducing new follicular emergence within a similar day range, but it results similar or greater P/FTAI.
本研究旨在比较苯甲酸雌二醇（EB）和 17β-雌二醇（E2）与孕激素（P4）联合用于定时人工授精（FTAI）方案的效果。我们假设 E2+P4 可更早地引发新的卵泡波（NFW），从而提高 FTAI 前的排卵前卵泡直径和妊娠率（P/FTAI）。实验 1 中，所有印度野牛（Bos indicus）奶牛（n=12/组）在第 0 天（D0）接受阴道内 P4 装置和 PGF2α 类似物剂量。D0 时，随机将母牛分为接受 EB 或 E2+P4 治疗的两组。在 D8.5，取出阴道内 P4 装置，并给所有母牛注射 PGF2α 和 EB 剂量，随后在 D10 进行固定时间人工授精。在 D0 和 D10 之间，通过卵巢超声检查确定主导卵泡的生长情况。在 D8.5 和 D10 评估主导卵泡壁中彩色功率多普勒信号的百分比。在实验 2 中，467 头母牛（2 岁初产[n=76]、经产[n=92]和多产[n=299]）接受了类似的 FTAI，并分为接受 EB（n=243）或 E2+P4（n=224）治疗。FTAI 后 30 天通过超声检查进行妊娠诊断。两种处理方式之间新卵泡波的出现时间相似（EB：3.7±0.37 vs. E2+P4：3.3±0.3，P=0.76）。接受 E2+P4 治疗的母牛在出现和 D9 之间的卵泡生长更大（P=0.06，1.18±0.07），而接受 EB 治疗的母牛生长较小（0.97±0.08）。E2+P4 对 D9 时的主导卵泡直径（13.0±0.6 比 10.9±0.55）和 D8.5 时卵泡壁的血液灌注也有积极影响（49 比 40%）。P/FTAI 存在治疗与胎次类别的交互作用效应（P<0.05）。E2+P4 治疗对初产奶牛的 P/FTAI 更有利（E2+P4：58%和 EB：30%）。然而，对于初产和多产奶牛，两种处理方式的 P/FTAI 相似（约 50%）。综上所述，在基于 E2/P4 的 FTAI 方案中，E2+P4 在诱导新卵泡出现方面与 EB 一样高效，且在相似的时间范围内，但它导致的 P/FTAI 相似或更高。
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