Department of Pharmacology, Ningxia Medical University, Yinchuan, 750004, China.
Key Laboratory of Hui Ethnic Medicine Modernization, The Ministry of Education, Ningxia Medical University, Yinchuan, 750004, China.
Chem Biol Interact. 2022 Oct 1;366:110144. doi: 10.1016/j.cbi.2022.110144. Epub 2022 Sep 5.
Spermatogenesis dysfunction is common in clinically infertile patients. Geniposide (GP) is one of the important active ingredients extracted from Eucommia ulmoides. However, the protective effect and mechanism of GP in the treatment of spermatogenic dysfunction is not known yet.
After cyclophosphamide-induced spermatogenic dysfunction was established in male mice, we gavaged GP for 4 weeks to evaluate spermatogenic function and anti-apoptotic effects by fertility, testicular weight, sperm quality, endoplasmic reticulum stress (ER stress), comet assay and serum testosterone level.
GP can improve the damage of fertility and reproductive organs induced by cyclophosphamide and increase the number and activity of sperm. In comet assay, it was found that GP administration could alleviate sperm DNA damage induced by cyclophosphamide. In addition, GP treatment can significantly reduce ThT fluorescence intensity and improve endoplasmic reticulum stress induced by cyclophosphamide. Besides, TUNEL staining and WB showed that GP could inhibit the excessive apoptosis of cells and protect testis. (p < 0.05, p < 0.01, p < 0.001).
The protective effect of Geniposide on cyclophosphamide-induced spermatogenic dysfunction in mice is related to the inhibition of endoplasmic reticulum stress.
生精功能障碍在临床上不育患者中很常见。京尼平苷（GP）是杜仲中提取的重要活性成分之一。然而，GP 治疗生精功能障碍的保护作用及其机制尚不清楚。
雄性小鼠经环磷酰胺诱导生精功能障碍后，给予 GP 灌胃 4 周，通过生育能力、睾丸重量、精子质量、内质网应激（ER 应激）、彗星试验和血清睾酮水平评价生精功能和抗凋亡作用。
GP 可改善环磷酰胺诱导的生育力和生殖器官损伤，增加精子数量和活力。在彗星试验中，发现 GP 给药可减轻环磷酰胺诱导的精子 DNA 损伤。此外，GP 治疗可显著降低 ThT 荧光强度，并改善环磷酰胺诱导的内质网应激。此外，TUNEL 染色和 WB 显示，GP 可抑制细胞过度凋亡，保护睾丸。（p<0.05，p<0.01，p<0.001）。
京尼平苷对环磷酰胺诱导的小鼠生精功能障碍的保护作用与抑制内质网应激有关。
