Key Laboratory of Health Risk Appraisal for Trace Toxic Chemicals of Zhejiang Province, Ningbo Municipal Center for Disease Control and Prevention, Ningbo, Zhejiang, 315010, China; Ningbo Key Laboratory of Poison Research and Control, Ningbo Municipal Center for Disease Control and Prevention, Ningbo, 315010, China.
Key Laboratory of Health Risk Appraisal for Trace Toxic Chemicals of Zhejiang Province, Ningbo Municipal Center for Disease Control and Prevention, Ningbo, Zhejiang, 315010, China; Ningbo Key Laboratory of Poison Research and Control, Ningbo Municipal Center for Disease Control and Prevention, Ningbo, 315010, China.
Environ Pollut. 2022 Nov 1;312:120092. doi: 10.1016/j.envpol.2022.120092. Epub 2022 Sep 2.
To date, knowledge of internal human exposure to BPA and its analogues (particularly bisphenol S and bisphenol F, etc.) remains limited. In the present study, a method involving dispersive solid-phase extraction and LC/MS was proposed to investigate the contamination levels of 28 precursor bisphenols and 9 major metabolites in serum. The critical variables of preparation method were screened out by Plackett-Burman design and further optimized by central composite design. Left in optimal conditions, a total of 286 samples consisting of 153 males and 133 females were analyzed. The results showed that BPA dominated over all the cases with the highest positive rate (82.2% of all the surveyed people), and totally four metabolites (BPA β-D-glucuronide, BPA monosulfate, BPA bis-(β-D-glucuronide) and BPS monosulfate) were detectable. The occurrence of BPA bis-(β-D-glucuronide) in serum is reported for the first time and its higher positive rate and contamination concentrations suggested that it may be a more important metabolite of BPA than others. Negligible potential risk of health effects to blood donors was observed, since the estimated exposure levels (mean 32.1 ng/kg bw/day, 95th 123.2 ng/kg bw/day) were well below far less than the temporary tolerable reference dose of BPA that recommended by the European Food Safety Authority (4 μg/kg bw/day by). The reference level of BPA for healthy population was determined to be 4.09 μg/L via the percentile method.
迄今为止，人们对于内源性人体接触双酚 A 及其类似物（尤其是双酚 S 和双酚 F 等）的了解仍然有限。在本研究中，提出了一种涉及分散固相萃取和 LC/MS 的方法，用于研究血清中 28 种前体双酚和 9 种主要代谢物的污染水平。通过 Plackett-Burman 设计筛选出制备方法的关键变量，并进一步通过中心复合设计进行优化。在最佳条件下，共分析了 286 份样本，其中包括 153 名男性和 133 名女性。结果表明，双酚 A 占所有情况的主导地位，阳性率最高（所有调查人群的 82.2%），总共检测到 4 种代谢物（BPAβ-D-葡糖苷酸、BPA 单硫酸盐、BPA 双-（β-D-葡糖苷酸）和 BPS 单硫酸盐）。首次报道了血清中 BPA 双-（β-D-葡糖苷酸）的存在，其较高的阳性率和污染浓度表明其可能是比其他代谢物更重要的 BPA 代谢物。由于估计的暴露水平（平均 32.1ng/kg bw/day，95%为 123.2ng/kg bw/day）远低于欧洲食品安全局推荐的 BPA 临时耐受参考剂量（4μg/kg bw/day），因此对献血者的健康影响几乎没有潜在风险。通过百分位法确定健康人群的 BPA 参考水平为 4.09μg/L。
