Tuzimski Tomasz, Szubartowski Szymon, Stążka Janusz, Baczewski Kamil, Janiszewska Daria, Railean Viorica, Buszewski Bogusław, Szultka-Młyńska Małgorzata
Department of Physical Chemistry, Faculty of Pharmacy, Medical University of Lublin, Chodźki 4a, 20-093 Lublin, Poland.
Doctoral School of Medical University of Lublin, Medical University of Lublin, Chodźki 6, 20-093 Lublin, Poland.
Molecules. 2025 Jan 1;30(1):140. doi: 10.3390/molecules30010140.
Bisphenols may negatively impact human health. In this study, we propose the use of HPLC-FLD for the simultaneous determination of bisphenols in pericardial fluid samples collected from patients with coronary artery disease undergoing coronary artery bypass surgery. For sample preparation, a fast, simple, and "green" DLLME method was used, achieving mean recovery values in the range of 62%-98% with relative standard deviations between 2% and 6% for all analytes. Quantitative analysis of bisphenols in the samples was then performed by LC-MS/MS on a triple quadrupole (QqQ) mass spectrometer and electrospray ionization (ESI-/ESI+) was applied in the negative and positive ion modes, respectively. The LODs and LOQs ranged from 0.04 ng/mL to 0.37 ng/mL and 0.12 ng/mL to 1.11 ng/mL, respectively. Pericardial fluid was collected from patients with coronary artery disease during coronary artery bypass surgery. Bisphenol residues were identified and quantified in samples from 19 patients. The procedure was successfully applied to the biomonitoring of free forms of 14 bisphenols in pericardial fluid. After statistical examination of the relationships between the selected variables, a strongly positive correlation was found between creatinine kinase and troponin I, as well as the number of venous anastomoses, circulation time, and clamp cap time.
双酚可能会对人体健康产生负面影响。在本研究中，我们建议使用高效液相色谱-荧光检测法（HPLC-FLD）同时测定从接受冠状动脉搭桥手术的冠心病患者采集的心包液样本中的双酚。对于样品制备，使用了一种快速、简单且“绿色”的分散液液微萃取（DLLME）方法，所有分析物的平均回收率在62%至98%之间，相对标准偏差在2%至6%之间。然后在三重四极杆（QqQ）质谱仪上通过液相色谱-串联质谱（LC-MS/MS）对样品中的双酚进行定量分析，并分别在负离子模式和正离子模式下应用电喷雾电离（ESI-/ESI+）。检测限（LOD）和定量限（LOQ）分别为0.04 ng/mL至0.37 ng/mL和0.12 ng/mL至1.11 ng/mL。在冠状动脉搭桥手术期间从冠心病患者采集心包液。在19名患者的样本中鉴定并定量了双酚残留。该方法成功应用于心包液中14种双酚游离形式的生物监测。在对所选变量之间的关系进行统计检验后，发现肌酸激酶与肌钙蛋白I之间以及静脉吻合数量、循环时间和夹闭时间之间存在强正相关。
