骨桥蛋白-5 剪接变体在小鼠原发性和转移性乳腺癌细胞中的表达。
Expression of osteopontin-5 splice variant in the mouse primary and metastatic breast cancer cells.
机构信息
Department of Tissue Engineering, School of Medicine, Shahroud University of Medical Sciences, Shahroud, Iran.
Department of Medical Laboratory Sciences, School of Allied Medical Sciences, Shahroud University of Medical Sciences, Shahroud, Iran.
出版信息
BMC Res Notes. 2022 Sep 5;15(1):286. doi: 10.1186/s13104-022-06179-w.
OBJECTIVE
Osteopontin (OPN) is a well-known glycoprotein involved in numerous pathobiological processes, including cancer. Despite having five splice variants for osteopontin in mice, the main focus of most studies has been on total OPN (tOPN). There are some studies on other splice variants, but the expression of osteopontin-5 (OPN5) has not been addressed in mouse cancer cells. Therefore, this study sought to evaluate OPN5 expression in mouse breast cancer cells.
RESULTS
The expression of OPN5 in primary and metastatic breast cancer cells of mice was confirmed in our study. These findings provided important insights regarding the OPN alternative splicing in mice for the first time. It is concluded that, like other OPN-SVs, OPN5 probably plays an essential role in tumor progression, which requires further investigation in different tumor models.
目的
骨桥蛋白（OPN）是一种参与多种病理生物过程的知名糖蛋白，包括癌症。尽管小鼠的骨桥蛋白有五种剪接变体，但大多数研究的主要关注点仍在总骨桥蛋白（tOPN）上。也有一些关于其他剪接变体的研究，但在小鼠癌细胞中尚未涉及骨桥蛋白-5（OPN5）的表达。因此，本研究旨在评估小鼠乳腺癌细胞中 OPN5 的表达。
结果
本研究证实了 OPN5 在小鼠原发性和转移性乳腺癌细胞中的表达。这些发现首次为我们提供了有关小鼠骨桥蛋白选择性剪接的重要见解。结论是，与其他 OPN-SVs 一样，OPN5 可能在肿瘤进展中发挥重要作用，这需要在不同的肿瘤模型中进一步研究。
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