Veres Katalin, Sinigla Mónika, Szabó Krisztina, Varga Nóra, Farkas Edit
Institute of Ecology and Botany, Centre for Ecological Research, Alkotmány u. 2-4, Vácrátót, H-2163 Hungary.
Bakony Museum of the Hungarian Natural History Museum, Rákóczi tér 3-5, Zirc, H-8420 Hungary.
Mycol Prog. 2022;21(9):83. doi: 10.1007/s11557-022-01831-y. Epub 2022 Sep 1.
Terricolous lichens are abundant in semi-arid areas, where they are exposed to high irradiation. Photoprotection is essential for the algae as the photobiont provides the primer carbon source for both symbionts. The UV-protectant lichen metabolites and different quenching procedures of the alga ensure adequate photoprotection. Since the long-term effect of diminishing UV-protectant lichen metabolites is unknown, a major part of lichen secondary metabolites was removed from thalli by acetone rinsing, and the lichens were then maintained under field conditions to investigate the effect on both symbionts for 3 years. Our aim was to determine if the decreased level of UV-protectant metabolites caused an elevated photoprotection in the algae and to reveal the dynamics of production of the metabolites. Photosynthetic activity and light protection were checked by chlorophyll fluorescence kinetics measurements every 6 months. The concentrations of fumarprotocetraric and usnic acids were monitored by chromatographic methods. Our results proved that seasonality had a more pronounced effect than that of acetone treatment on the function of lichens over a long-term scale. Even after 3 years, the acetone-treated thalli contained half as much usnic acid as the control thalli, and the level of photoprotection remained unchanged in the algae. However, the amount of available humidity was a more critical limiting environmental factor than the amount of incoming irradiation affecting usnic acid production. The lichenicolous fungus became relatively more abundant in the acetone-treated samples than in the control samples, indicating a slight change caused by the treatment.
The online version contains supplementary material available at 10.1007/s11557-022-01831-y.
土生地衣在半干旱地区很丰富，在那里它们受到高辐射。光保护对藻类至关重要，因为光合生物为两种共生体提供主要碳源。紫外线防护地衣代谢产物和藻类的不同淬灭程序可确保充分的光保护。由于紫外线防护地衣代谢产物减少的长期影响尚不清楚，通过丙酮冲洗从地衣体中去除了大部分地衣次生代谢产物，然后将地衣置于田间条件下3年，以研究对两种共生体的影响。我们的目的是确定紫外线防护代谢产物水平的降低是否会导致藻类光保护的提高，并揭示代谢产物的产生动态。每6个月通过叶绿素荧光动力学测量检查光合活性和光保护。通过色谱方法监测富马原岛衣酸和松萝酸的浓度。我们的结果证明，从长期来看，季节性对地衣功能的影响比丙酮处理更为明显。即使在3年后，经丙酮处理的地衣体中松萝酸的含量仍只有对照地衣体的一半，而藻类中的光保护水平保持不变。然而，有效湿度量是比影响松萝酸产生的入射辐射量更关键的限制环境因素。在经丙酮处理的样品中，地衣内生真菌比对照样品中相对更为丰富，表明该处理引起了轻微变化。
在线版本包含可在10.1007/s11557-022-01831-y获取的补充材料。
