-Cyfluthrin-Mediated Cytotoxicity of Cultured Rat Primary Hepatocytes Ameliorated by Cotreatment with Luteolin.

The current study was designed to evaluate the possible protective effects of luteolin against -cyfluthrin-mediated toxicity on the primary culture of rat hepatocytes (RHs). In the first step, the exposure of RHs to -cyfluthrin (10, 20, 40, and 80 M) was assessed by MTT. Second, redox condition was evaluated in cotreatment of cells with luteolin (20, 40, and 60 M) and -cyfluthrin (40 M) at both medium and intra levels. In comparison to control, viability was lower in 40 and 80 M -cyfluthrin-treated groups at 24 h and all -cyfluthrin-treated groups at 48 h ( < 0.05). Cotreatment with 20 or 40 M luteolin + 40 M -cyfluthrin resulted in a higher viability value compared to -cyfluthrin alone at 24 and 48 h of incubation ( < 0.05). Administration of 20 or 40 M luteolin with -cyfluthrin led to the decrease of malondialdehyde and total nitrate/nitrite and the increase of total antioxidant capacity (TAC) values in both medium and intrahepatocyte levels compared to the -cyfluthrin-treated group at 48 h ( < 0.05). It seems that low and medium doses of luteolin possess the potential to reduce -cyfluthrin-mediated hepatotoxicity via attenuation of peroxidative/nitrosative reactions and augmentation of TAC levels.