Wang Kun, Bao Jun-Ping, Zhou Zhi-Min, Mao Lu, Wu Xiao-Tao
Department of Orthopedics, Zhongda Hospital, Southeast University, Nanjing, China.
Medical School of Southeast University, Nanjing, China.
J Oncol. 2022 Aug 27;2022:7246904. doi: 10.1155/2022/7246904. eCollection 2022.
Increasing evidence has shown that noncoding RNAs perform a remarkable function in neuropathic pain (NP); nonetheless, the mechanisms underlying the modulation of competitive endogenous RNA in NP remain uncertain. The goal of this research was to investigate the molecular processes underlying NP.
We utilized the Gene Expression Omnibus (GEO) to obtain NP-related microarray datasets that included the expression patterns of circular RNAs (circRNAs) and messenger RNAs (mRNAs). Following that, bioinformatics analyses and a molecular biology experiment were carried out.
According to the findings, carrying out enrichment studies of the targeted genes had an impact on a variety of NP-related pathways. Notably, we isolated a ceRNA subnetwork incorporating two upregulated circRNAs (Esrrg and Map3k3) which primarily participate in the focal adhesion pathway by regulating Integrin Subunit Beta 4 (ITGB4) and two downregulated circRNAs (Dgkb and Atp2a2), which potentially regulate metabolism-related molecule Lipase A (LIPA).
According to our findings, the focal adhesion and metabolic signaling pathways could be critical in the advancement of NP, and some circRNA might regulate this biological process through the ceRNA network, which might offer pertinent insights into the underlying mechanisms.
越来越多的证据表明，非编码RNA在神经性疼痛（NP）中发挥着重要作用；然而，NP中竞争性内源RNA调控的潜在机制仍不明确。本研究的目的是探究NP背后的分子机制。
我们利用基因表达综合数据库（GEO）获取与NP相关的微阵列数据集，其中包括环状RNA（circRNA）和信使RNA（mRNA）的表达模式。随后，进行了生物信息学分析和分子生物学实验。
根据研究结果，对靶向基因进行富集研究对多种NP相关途径产生了影响。值得注意的是，我们分离出一个ceRNA子网，其中包含两个上调的circRNA（Esrrg和Map3k3），它们主要通过调节整合素亚基β4（ITGB4）参与粘着斑途径，以及两个下调的circRNA（Dgkb和Atp2a2），它们可能调节与代谢相关的分子脂肪酶A（LIPA）。
根据我们的研究结果，粘着斑和代谢信号通路可能在NP的进展中起关键作用，一些circRNA可能通过ceRNA网络调节这一生物学过程，这可能为潜在机制提供相关见解。
