Pereira Rafaela Vaz Sousa, Ugarte-Berzal Estefania, Vandooren Jennifer, Nylander Karin, Martens Erik, Van Mellaert Lieve, Van Damme Jo, Vranckx Jan Jeroen, Matthys Patrick, Alamäe Tiina, Phillipson Mia, Visnapuu Triinu, Opdenakker Ghislain
Department of Microbiology, Immunology and Transplantation, Rega Institute for Medical Research, KU Leuven, Leuven, Belgium.
Department of Medical Cell Biology, Division of Integrative Physiology, Uppsala University, Uppsala, Sweden.
J Inflamm Res. 2022 Aug 30;15:4995-5008. doi: 10.2147/JIR.S375487. eCollection 2022.
To verify the antibacterial and immunomodulatory effects of the amylose derivative - chlorite-oxidized oxyamylose (COAM) - in a skin wound setting.
In vitro antibacterial effects of COAM against opportunistic bacterial pathogens common to skin wounds, including and methicillin-resistant (MRSA), were determined by cultivation methods. The effects of COAM on myeloid cell infiltration into full thickness skin wounds were investigated in wild-type and in transgenic CXCR1-GFP mice.
On the basis of in vitro experiments, an antibacterial effect of COAM against species including MRSA was confirmed. The minimum inhibitory concentration of COAM was determined as 2000 µg/mL against these bacterial strains. Control full thickness skin wounds yielded maximal neutrophil influxes and no additive effect on neutrophil influx was observed following topical COAM-treatment. However, COAM administration increased local CXCR1 macrophage counts at days 3 and 4 and induced a trend towards better wound healing.
Aside from its known broad antiviral impact, COAM possesses in vitro antibacterial effects specifically against Gram-positive opportunistic pathogens of the skin and modulates in vivo macrophage contents in mouse skin wounds.
验证直链淀粉衍生物——亚氯酸盐氧化氧化直链淀粉（COAM）——在皮肤伤口环境中的抗菌和免疫调节作用。
通过培养方法测定COAM对皮肤伤口常见的机会性细菌病原体的体外抗菌作用，这些病原体包括[具体细菌名称缺失]和耐甲氧西林金黄色葡萄球菌（MRSA）。在野生型和转基因CXCR1-GFP小鼠中研究了COAM对髓样细胞浸润全层皮肤伤口的影响。
基于体外实验，证实了COAM对包括MRSA在内的多种细菌具有抗菌作用。COAM对这些细菌菌株的最低抑菌浓度确定为2000μg/mL。对照全层皮肤伤口产生了最大的中性粒细胞流入，局部应用COAM处理后未观察到对中性粒细胞流入的附加作用。然而，在第3天和第4天给予COAM增加了局部CXCR1巨噬细胞数量，并诱导了伤口愈合更好的趋势。
除了其已知的广泛抗病毒作用外，COAM具有体外抗菌作用，特别是针对皮肤的革兰氏阳性机会性病原体，并调节小鼠皮肤伤口中的体内巨噬细胞含量。
