Tris和Hepes对氨甲酰磷酸合酶（氨）的抑制作用。对N-乙酰谷氨酸解离常数（Ka）的影响。
Inhibition of carbamoyl-phosphate synthase (ammonia) by Tris and Hepes. Effect on Ka for N-acetylglutamate.
作者信息
Lund P, Wiggins D
出版信息
Biochem J. 1987 Apr 1;243(1):273-6. doi: 10.1042/bj2430273.
Abstract
The apparent Ka for N-acetylglutamate of rat liver carbamoyl-phosphate synthase is 11 microM in phosphate buffer, a value 10-fold lower than reported in other buffer systems. Tris and Hepes inhibit competitively with N-acetylglutamate. The proportion of carbamoyl-phosphate synthase in the active enzyme-acetylglutamate complex in vivo may be higher than previous calculations suggest, which re-opens the question of the involvement of N-acetylglutamate in the regulation of urea synthesis.
摘要
大鼠肝脏氨甲酰磷酸合成酶的N - 乙酰谷氨酸表观解离常数（Ka）在磷酸盐缓冲液中为11微摩尔，该值比在其他缓冲系统中报道的低10倍。Tris和Hepes与N - 乙酰谷氨酸竞争性抑制。体内活性酶 - 乙酰谷氨酸复合物中氨甲酰磷酸合成酶的比例可能高于先前计算结果所显示的，这重新引发了N - 乙酰谷氨酸参与尿素合成调节的问题。
相似文献
1
Inhibition of carbamoyl-phosphate synthase (ammonia) by Tris and Hepes. Effect on Ka for N-acetylglutamate.Tris和Hepes对氨甲酰磷酸合酶（氨）的抑制作用。对N-乙酰谷氨酸解离常数（Ka）的影响。
Biochem J. 1987 Apr 1;243(1):273-6. doi: 10.1042/bj2430273.
2
Influence of anions on the activation of carbamoyl phosphate synthetase (ammonia) by acetylglutamate: implications for the activation of the enzyme in the mitochondria.
Arch Biochem Biophys. 1991 Aug 1;288(2):414-20. doi: 10.1016/0003-9861(91)90214-4.
3
Differential effects of N-acetylglutamate on citrulline synthesis by coupled and uncoupled mitochondria.N-乙酰谷氨酸对耦联和非耦联线粒体合成瓜氨酸的不同作用。
Arch Biochem Biophys. 1984 Oct;234(1):31-44. doi: 10.1016/0003-9861(84)90321-7.
4
The mechanism of Ca2+ stimulation of citrulline and N-acetylglutamate synthesis by mitochondria.线粒体对瓜氨酸和N-乙酰谷氨酸合成的Ca2+刺激机制。
Biochim Biophys Acta. 1990 Jan 29;1033(1):85-90. doi: 10.1016/0304-4165(90)90198-6.
5
The apparent Km of ammonia for carbamoyl phosphate synthetase (ammonia) in situ.氨甲酰磷酸合成酶（氨）原位时氨的表观米氏常数。
Biochem J. 1985 Jul 1;229(1):205-11. doi: 10.1042/bj2290205.
6
Properties of carbamoyl-phosphate synthetase (ammonia) in rat-liver mitochondria made permeable with toluene.甲苯使大鼠肝脏线粒体通透后氨甲酰磷酸合成酶（氨）的性质
Eur J Biochem. 1983 Sep 15;135(2):251-8. doi: 10.1111/j.1432-1033.1983.tb07645.x.
7
The relationship between intramitochondrial N-acetylglutamate and activity of carbamoyl-phosphate synthetase (ammonia). The effect of glucagon.线粒体内N-乙酰谷氨酸与氨甲酰磷酸合成酶（氨）活性之间的关系。胰高血糖素的作用。
Eur J Biochem. 1980;107(1):197-205. doi: 10.1111/j.1432-1033.1980.tb04640.x.
8
Inactivation of mitochondrial carbamoyl phosphate synthetase induced by ascorbate, oxygen, and Fe3+ in the presence of acetylglutamate: protection by ATP and HCO3- and lack of inactivation of ornithine transcarbamylase.在乙酰谷氨酸存在的情况下，抗坏血酸、氧气和Fe3+诱导线粒体氨甲酰磷酸合成酶失活：ATP和HCO3-的保护作用以及鸟氨酸转氨甲酰酶未失活
Arch Biochem Biophys. 1987 Nov 1;258(2):342-50. doi: 10.1016/0003-9861(87)90353-5.
9
Does ornithine stimulate carbamoylphosphate synthetase?鸟氨酸是否刺激氨甲酰磷酸合成酶？
Int J Biochem. 1985;17(4):503-7. doi: 10.1016/0020-711x(85)90146-6.
10
Synthesis of carbamoyl phosphate by carbamoyl phosphate synthetase I in the absence of acetylglutamate. Activation of the enzyme by cryoprotectants.在缺乏乙酰谷氨酸的情况下，由氨甲酰磷酸合成酶I合成氨甲酰磷酸。冷冻保护剂对该酶的激活作用。
Biochem Biophys Res Commun. 1981 Apr 30;99(4):1131-7. doi: 10.1016/0006-291x(81)90736-1.
引用本文的文献
1
Parameter Reliability and Understanding Enzyme Function.参数可靠性与酶功能理解。
Molecules. 2022 Jan 1;27(1):263. doi: 10.3390/molecules27010263.
2
Authors' Reply: Pyridine nucleotide regulation of hepatic endoplasmic reticulum calcium uptake.作者回复：吡啶核苷酸对肝内质网钙摄取的调节
Physiol Rep. 2019 Oct;7(19):e14258. doi: 10.14814/phy2.14258.
3
Down-regulation of hepatic urea synthesis by oxypurines: xanthine and uric acid inhibit N-acetylglutamate synthase.黄嘌呤和尿酸通过下调肝尿素合成：抑制 N-乙酰谷氨酸合成酶。
J Biol Chem. 2011 Jun 24;286(25):22055-68. doi: 10.1074/jbc.M110.209023. Epub 2011 May 3.
4
Effect of HEPES buffer on the uptake and transport of P-glycoprotein substrates and large neutral amino acids.HEPES 缓冲液对 P-糖蛋白底物和大中性氨基酸摄取和转运的影响。
Mol Pharm. 2010 Apr 5;7(2):412-20. doi: 10.1021/mp900193e.
5
Regulation of urea and citrulline synthesis under physiological conditions.生理条件下尿素和瓜氨酸合成的调节
Biochem J. 1993 May 15;292 ( Pt 1)(Pt 1):241-7. doi: 10.1042/bj2920241.
6
Effect of level of dietary protein on arginine-stimulated citrulline synthesis. Correlation with mitochondrial N-acetylglutamate concentrations.膳食蛋白质水平对精氨酸刺激的瓜氨酸合成的影响。与线粒体N-乙酰谷氨酸浓度的相关性。
Biochem J. 1990 Dec 15;272(3):671-5. doi: 10.1042/bj2720671.
本文引用的文献
1
Functions of aldehyde reductases.醛还原酶的功能。
Biochem Soc Trans. 1981 Aug;9(4):279-81. doi: 10.1042/bst0090279.
2
N-acetylglutamate-independent activity of carbamyl phosphate synthetase (ammonia): implications for the kinetic assay of acetylglutamate.氨甲酰磷酸合成酶（氨）的N-乙酰谷氨酸非依赖性活性：对乙酰谷氨酸动力学测定的影响
Arch Biochem Biophys. 1984 Jul;232(1):38-46. doi: 10.1016/0003-9861(84)90519-8.
3
Is N-acetylglutamate a short-term regulator of urea synthesis?N-乙酰谷氨酸是尿素合成的短期调节因子吗？
Biochem J. 1984 Mar 15;218(3):991-4. doi: 10.1042/bj2180991.
4
N-acetylglutamate and urea synthesis.N-乙酰谷氨酸与尿素合成。
Biochem J. 1984 Oct 15;223(2):559-60. doi: 10.1042/bj2230559.
5
Female sex hormone and nephropathy in Cohen diabetic rat (genetically selected sucrose-fed).
Horm Metab Res. 1984 Jan;16(1):11-6. doi: 10.1055/s-2007-1014683.
6
Human carbamylphosphate synthetase I. Stabilization, purification, and partial characterization of the enzyme from human liver.人氨甲酰磷酸合成酶I。人肝脏中该酶的稳定性、纯化及部分特性鉴定
J Biol Chem. 1980 Aug 25;255(16):7891-5.
7
Short term regulation of ureagenesis.尿素生成的短期调节
J Biol Chem. 1980 Jun 10;255(11):5270-80.
8
Kinetic studies on rat liver carbamyl phosphate synthetase.大鼠肝脏氨甲酰磷酸合成酶的动力学研究。
J Biol Chem. 1968 Aug 25;243(16):4279-85.
9
Kinetic studies of bovine liver carbamoyl phosphate synthetase.牛肝氨甲酰磷酸合成酶的动力学研究
Biochem J. 1974 Sep;141(3):807-16. doi: 10.1042/bj1410807.
10
Stabilization of mitochondrial functions with digitonin.
Methods Enzymol. 1979;55:115-8. doi: 10.1016/0076-6879(79)55016-2.
Zotero 插件