Jafari Hasan, Hemmati Seyed Ali, Habibpour Behzad
Department of Plant Protection, Faculty of Agriculture, Shahid Chamran University of Ahvaz, Ahvaz, Iran.
Bull Entomol Res. 2023 Feb;113(1):133-143. doi: 10.1017/S0007485322000402. Epub 2022 Sep 6.
(Hübner) is considered a serious agricultural pest worldwide. We explored the effects of artificial diets containing ten legumes, including broad beans (Shadan, Feyz, Saraziri, Barekat, and Mahta cultivars), white kidney beans (Dehghan cultivar), red kidney beans (Goli cultivar), common beans (Khomein cultivar), cowpeas (Mashhad and Arabi cultivars) on the feeding responses of by quantifying specific primary and secondary metabolites in the studied legumes and determining larval nutritional indices and digestive enzyme activities. The results showed that the highest efficiency of conversion of digested food (ECD) and relative growth rate values (RGR) of whole larval instars were obtained in the Dehghan and Goli cultivars. However, the lowest values of ECD and RGR were observed in the larvae fed on the Khomein and Mahta cultivars. The highest proteolytic and amylolytic activities of larvae were found on the Dehghan and Mashhad cultivars. The highest and lowest values of standardized insect-growth index and index of plant quality were observed in larvae feeding on the Dehghan and Khomein cultivars, respectively. Additionally, significant variations in phytochemical metabolites were recorded among the studied legume cultivars. Significant negative or positive correlations were also found between feeding characteristics and enzymatic activities of with the biochemical composition of the studied legumes. The cluster analysis results revealed that artificial diets containing Mahta and Khomein cultivars were unsuitable for , and can be used as candidates for integrated pest management programs or for screening insect inhibitors to produce genetically modified pest-resistant plants.
（胡伯纳）在全球范围内被视为一种严重的农业害虫。我们通过量化所研究豆类中的特定初级和次级代谢产物，并测定幼虫营养指标和消化酶活性，探索了包含十种豆类的人工饲料对其取食反应的影响，这十种豆类包括蚕豆（沙丹、费兹、萨拉齐里、巴雷卡特和马赫塔品种）、白芸豆（德赫甘品种）、红芸豆（戈利品种）、普通豆（霍梅因品种）、豇豆（马什哈德和阿拉伯品种）。结果表明，在德赫甘和戈利品种中，整个幼虫龄期的消化食物转化率（ECD）和相对生长率（RGR）值最高。然而，在以霍梅因和马赫塔品种为食的幼虫中，ECD和RGR值最低。在德赫甘和马什哈德品种上，幼虫的蛋白水解和淀粉水解活性最高。在以德赫甘品种为食的幼虫中，标准化昆虫生长指数和植物质量指数分别出现最高和最低值。此外，在所研究的豆类品种中，植物化学代谢产物存在显著差异。在所研究豆类的生化组成与的取食特性和酶活性之间也发现了显著的负相关或正相关。聚类分析结果表明，含有马赫塔和霍梅因品种的人工饲料不适用于，可作为综合害虫管理计划的候选方案或用于筛选昆虫抑制剂以培育转基因抗虫植物。
