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棉铃虫(Helicoverpa armigera)对七个受试菜豆品种的营养表现及某些消化酶活性

Nutritional performance and activity of some digestive enzymes of the cotton bollworm, Helicoverpa armigera, in response to seven tested bean cultivars.

作者信息

Namin Foroogh Rahimi, Naseri Bahram, Razmjou Jabraeil

机构信息

Department of Plant Protection, Faculty of Agriculture, University of Mohaghegh Ardabili, Ardabil, Iran

出版信息

J Insect Sci. 2014;14:93. doi: 10.1093/jis/14.1.93.

Abstract

Nutritional performance and activity of some digestive enzymes (protease and α-amylase) of Helicoverpa armigera Hübner (Lepidoptera: Noctuidae) in response to feeding on bean (Phaseolus vulgaris L. (Fabales: Fabaceae)) cultivars (Shokufa, Akhtar, Sayyad, Naz, Pak, Daneshkadeh, and Talash) were evaluated under laboratory conditions (25 ± 1°C, 65 ± 5% RH, and a 16:8 L:D photoperiod). The highest and lowest respective values of approximate digestibility were observed when fourth, fifth, and sixth larval instar H. armigera were fed red kidney bean Akhtar and white kidney bean Daneshkadeh. The efficiency of conversion of ingested and digested food was highest when H. armigera was fed red kidney beans Akhtar and Naz and lowest when they were fed white kidney bean Pak. The highest protease activity of fifth instars was observed when they were fed red kidney bean Naz, and the highest amylase activity of fifth instars was observed when they were fed red kidney bean Sayyad. Sixth instar larvae that fed on red kidney bean Sayyad showed the highest protease activity. Larvae reared on common bean Talash and white kidney bean Pak showed the highest amylase activity. Among bean cultivars tested, red kidney bean Sayyad was the most unsuitable host for feeding H. armigera.

摘要

在实验室条件(25±1°C、65±5%相对湿度和16:8光暗周期)下,评估了棉铃虫(鳞翅目:夜蛾科)取食不同菜豆(菜豆属:豆科)品种(Shokufa、Akhtar、Sayyad、Naz、Pak、Daneshkadeh和Talash)后某些消化酶(蛋白酶和α-淀粉酶)的营养性能和活性。当棉铃虫四龄、五龄和六龄幼虫取食红芸豆Akhtar和白芸豆Daneshkadeh时,分别观察到近似消化率的最高值和最低值。当棉铃虫取食红芸豆Akhtar和Naz时,摄入食物和消化食物的转化效率最高;当取食白芸豆Pak时,转化效率最低。五龄幼虫取食红芸豆Naz时,蛋白酶活性最高;五龄幼虫取食红芸豆Sayyad时,淀粉酶活性最高。取食红芸豆Sayyad的六龄幼虫蛋白酶活性最高。以普通菜豆Talash和白芸豆Pak饲养的幼虫淀粉酶活性最高。在所测试的菜豆品种中,红芸豆Sayyad是最不适合棉铃虫取食的寄主。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/825f/4212854/b114e80d4f32/jis-14-1-0093-fig1.jpg

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