University of Medicine and Pharmacy, Vietnam National University, Hanoi, Vietnam.
College of Pharmacy, Gachon University, Incheon, Republic of Korea.
Phytother Res. 2023 Jan;37(1):140-150. doi: 10.1002/ptr.7602. Epub 2022 Sep 6.
Huperzia serrata contains Huperzine A (HupA)-an alkaloid used to treat cognitive dysfunction. In this study, we used the total alkaloids (HsAE) to investigate their potential in managing cognitive impairment in comparison with HupA. The antioxidant activity was measured by DPPH assay. In the cellular study, the cell viability and level of ACh of SH-SY5Y cells were evaluated after pretreated with HsAE and scopolamine. For in vivo assay, mice were pre-treated with HsAE, and HupA and undergone scopolamine injection for cognitive impairment. The behavioral tests including the Y-maze and Morris water maze test and the AChE activity, the SOD, CAT, MDA level in the hippocampus and cortex were evaluated. HsAE showed significant scavenging properties on DPPH radicals. HsAE was not toxic to SH-SY5Y cells, and can rescue these cells upon scopolamine treatment. Intriguingly, HsAE showed the neuroprotection against scopolamine-induced amnesia in mice. Moreover, HsAE decreased AChE activity, MDA level, increased antioxidative enzyme activity in the hippocampus as well as cortex of mice, which was relatively better than that of HupA. These findings suggested that HsAE may significantly protect the neurons of mice with scopolamine-induced memory impairment connected to AChE depletion and oxidative stress.
石杉碱甲（HupA）是一种用于治疗认知功能障碍的生物碱，而石杉含有石杉碱甲（HupA）。在这项研究中，我们使用总生物碱（HsAE）来研究其在治疗认知障碍方面的潜力，将其与石杉碱甲进行比较。通过 DPPH 法测定抗氧化活性。在细胞研究中，通过 DPPH 法测定细胞活力和 ACh 水平，评估 HsAE 和东莨菪碱预处理 SH-SY5Y 细胞后的活力和 ACh 水平。在体内实验中，用 HsAE、石杉碱甲预处理小鼠，然后用东莨菪碱注射进行认知障碍。评估行为测试（Y 迷宫和 Morris 水迷宫测试）和 AChE 活性、海马和皮质中的 SOD、CAT、MDA 水平。HsAE 对 DPPH 自由基具有明显的清除作用。HsAE 对 SH-SY5Y 细胞没有毒性，并且可以在东莨菪碱处理后挽救这些细胞。有趣的是，HsAE 对东莨菪碱诱导的小鼠健忘症具有神经保护作用。此外，HsAE 降低了 AChE 活性、MDA 水平，增加了海马和皮质中的抗氧化酶活性，相对优于石杉碱甲。这些发现表明，HsAE 可能通过降低 AChE 活性和氧化应激，显著保护东莨菪碱诱导的记忆障碍小鼠的神经元。
