HNK-1表位（3-硫酸葡萄糖醛酸）在PC12嗜铬细胞瘤细胞、嗜铬粒细胞膜和硫酸软骨素蛋白聚糖中的存在情况。

Occurrence of the HNK-1 epitope (3-sulfoglucuronic acid) in PC12 pheochromocytoma cells, chromaffin granule membranes, and chondroitin sulfate proteoglycans.

作者信息

Margolis R K, Ripellino J A, Goossen B, Steinbrich R, Margolis R U

出版信息

Biochem Biophys Res Commun. 1987 Jun 30;145(3):1142-8. doi: 10.1016/0006-291x(87)91556-7.

Abstract

After biosynthetic labeling of sulfated glycoproteins in rat and goldfish brain and PC12 pheochromocytoma cells with sodium [35S]sulfate, it was observed that all of the bands reactive with the HNK-1 antibody on immunoblots of sodium dodecyl sulfate-polyacrylamide gels corresponded with sulfate-labeled proteins detected by fluorography. These results support data from other studies, which indicate that the HNK-1 epitope is a 3-sulfo-glucuronic acid residue. In addition to its presence in a wide range of nervous tissue glycoproteins, the HNK-1 epitope was also detected in chromaffin granule membranes, chondroitinase ABC, and in chondroitin sulfate proteoglycans of brain, cartilage, and chondrosarcoma. However, it is not present in the heparan sulfate proteoglycan of brain, or in either of two chondroitin sulfate/dermatan sulfate proteoglycans in the chromaffin granule matrix.

摘要

在用[35S]硫酸钠对大鼠、金鱼脑以及PC12嗜铬细胞瘤细胞中的硫酸化糖蛋白进行生物合成标记后，观察到在十二烷基硫酸钠-聚丙烯酰胺凝胶免疫印迹上与HNK-1抗体反应的所有条带，都与通过荧光自显影检测到的硫酸盐标记蛋白相对应。这些结果支持了其他研究的数据，这些数据表明HNK-1表位是一个3-磺基葡糖醛酸残基。除了存在于广泛的神经组织糖蛋白中，HNK-1表位还在嗜铬颗粒膜、软骨素酶ABC以及脑、软骨和软骨肉瘤的硫酸软骨素蛋白聚糖中被检测到。然而，它不存在于脑的硫酸乙酰肝素蛋白聚糖中，也不存在于嗜铬颗粒基质中的两种硫酸软骨素/硫酸皮肤素蛋白聚糖中的任何一种中。