牛肾上腺髓质嗜铬颗粒膜的神经节苷脂组成

Ganglioside composition of chromaffin granule membrane in bovine adrenal medulla.

作者信息

Sekine M, Ariga T, Miyatake T, Kuroda Y, Suzuki A, Yamakawa T

出版信息

J Biochem. 1984 Jan;95(1):155-60. doi: 10.1093/oxfordjournals.jbchem.a134579.

DOI:10.1093/oxfordjournals.jbchem.a134579

Abstract

The ganglioside composition of chromaffin granule membranes of bovine adrenal medulla was investigated. Two major gangliosides, which accounted for over 95% of total gangliosides, were characterized as GM3 (NeuAc) and GM3 (NeuGc) by thin layer chromatography (TLC) and gas-liquid chromatography (GLC). Chromaffin granule membranes contained a relatively higher concentration of gangliosides than the membranes of other subcellular organelles; three times higher than microsomal membranes and seven times higher than mitochondrial membranes. This high concentration of ganglioside in chromaffin granule membranes could contribute to the high concentration of gangliosides in the adrenal medulla as compared to other extraneural tissues.

摘要

对牛肾上腺髓质嗜铬颗粒膜的神经节苷脂组成进行了研究。通过薄层色谱法（TLC）和气液色谱法（GLC）鉴定出两种主要的神经节苷脂，它们占总神经节苷脂的95%以上，分别为GM3（NeuAc）和GM3（NeuGc）。嗜铬颗粒膜所含神经节苷脂的浓度相对高于其他亚细胞器膜；比微粒体膜高三倍，比线粒体膜高七倍。与其他神经外组织相比，嗜铬颗粒膜中这种高浓度的神经节苷脂可能导致肾上腺髓质中神经节苷脂浓度较高。