Sánchez-Yagüe J, Cabezas J A, Llanillo M
Biochem Biophys Res Commun. 1987 Jun 30;145(3):1362-7. doi: 10.1016/0006-291x(87)91588-9.
Phospholipid orientation in platelet plasma membranes and other blood cells, such as erythrocytes, appears to be rather similar. The negatively charged phospholipids are almost exclusively located on the inner leaflet of the bilayer. No phosphatidylserine is present on the outer membrane bilayer. The results of the present study, using a specific reagent for amino groups, trinitrobenzenesulfanilic acid, showed that in sheep platelet plasma membranes enriched with free exogenous cholesterol, an alteration in the aminophospholipid topology occurs, with a portion of phosphatidylserine moving from the inner to the outer side. A progressive appearance of aminophospholipids in the outer membrane bilayer was also observed in artificial vesicles prepared with total lipids from sheep platelets supplemented with increased amounts of free cholesterol.
血小板质膜以及其他血细胞（如红细胞）中的磷脂取向似乎颇为相似。带负电荷的磷脂几乎完全位于双层膜的内层。外膜双层中不存在磷脂酰丝氨酸。本研究使用一种针对氨基的特异性试剂——三硝基苯磺酸，结果表明，在富含游离外源性胆固醇的绵羊血小板质膜中，氨基磷脂拓扑结构发生改变，一部分磷脂酰丝氨酸从内层移至外层。在用补充了增加量游离胆固醇的绵羊血小板总脂质制备的人工囊泡中，也观察到外膜双层中氨基磷脂逐渐出现。
