Jain S K
J Biol Chem. 1984 Mar 25;259(6):3391-4.
The distribution of phospholipids across erythrocyte membrane bilayer is asymmetrical. The present study reports the effect of malonyldialdehyde (MDA), a product of fatty acid peroxidation, on the organization of phosphatidylserine (PS) and phosphatidylethanolamine (PE) in human erythrocytes using a nonpermeable bee venom phospholipase A2 and trinitrobenzene-sulfonilic acid. MDA accumulation in the erythrocytes was accomplished both by its increased endogenous generation after exposure of cells to H2O2, as well as by the treatment of erythrocytes with exogenous authentic MDA. The above treatments resulted in a significantly increased movement of PS and PE from inner bilayer to outer bilayer, which had a highly positive correlation with the concentration of MDA in the erythrocyte membranes. Antioxidants vitamin E, butylated hydroxytoluene, and butylated hydroxyanisole inhibited the effect of H2O2 treatment on erythrocyte membrane lipid organization by scavenging fatty acid peroxidation and formation of MDA. Thus, lipid peroxidation and MDA accumulation can disturb organization of PS and PE in the human erythrocyte membrane bilayer.
磷脂在红细胞膜双分子层中的分布是不对称的。本研究报道了脂肪酸过氧化产物丙二醛（MDA）对人红细胞中磷脂酰丝氨酸（PS）和磷脂酰乙醇胺（PE）组织的影响，采用了非渗透性的蜂毒磷脂酶A2和三硝基苯磺酸。红细胞中MDA的积累通过细胞暴露于H2O2后内源性生成增加以及用外源性纯MDA处理红细胞来实现。上述处理导致PS和PE从内双分子层向外双分子层的移动显著增加，这与红细胞膜中MDA的浓度高度正相关。抗氧化剂维生素E、丁基羟基甲苯和丁基羟基茴香醚通过清除脂肪酸过氧化和MDA的形成，抑制了H2O2处理对红细胞膜脂质组织的影响。因此，脂质过氧化和MDA积累会扰乱人红细胞膜双分子层中PS和PE的组织。
