Rudtanatip Tawut, Somintara Somsuda, Sakaew Waraporn, El-Abid Jamal, Cano Maria Emilia, Jongsomchai Kamonwan, Wongprasert Kanokpan, Kovensky José
Electron Microscopy Unit, Department of Anatomy, Faculty of Medicine, Khon Kaen University, Mueang, Khon Kaen, 40002, Thailand.
Laboratoire de Glycochimie, des Antimicrobiens et des Agroressources (LG2A) CNRS UMR 7378, Institut de Chimie de Picardie FR 3085, Université de Picardie Jules Verne, 33 rue Saint Leu, Amiens, 80039, France.
Macromol Biosci. 2022 Dec;22(12):e2200172. doi: 10.1002/mabi.202200172. Epub 2022 Sep 14.
Sulfated galactans (SG) isolated from Gracilaria fisheri is partially degraded (DSG), and subsequentially supplemented with octanoyl (DSGO) and sulfate (DSGS) groups. The molecular weights of DSG, DSGO, and DSGS are 7.87, 152.79, and 97.07 kDa, respectively. The modification is confirmed using FTIR and NMR, while in vitro wound healing activity is assessed using scratched wound fibroblasts. The results reveal that DSGO exhibits highest percentage of wound closure in scratched fibroblast L929 cells. Furthermore, DSGO is able to promote proliferation and accelerate migration of scratched fibroblasts, which correspond to the regulation of proteins and mRNA (Ki67, p-FAK, vimentin, and E-cadherin) determined by Western blotting and qPCR analysis. The superior wound healing activity of DSGO is also confirmed in excision wound of rats. The results demonstrate that DSGO significantly enhances the percentage of wound closure, re-epithelialization, and collagen arrangement, increases α-smoth muscle actin (α-SMA) and vimentin expression, and decreases that of tumor necrosis factor-α (TNF-α) at the wound site. The results suggest that degraded SG supplemented with medium-chain fatty acids of octanoyl group may pass through the membrane, subsequently activating the mediators associated with proliferation and migration of fibroblasts, which can potentially lead to the promotion of wound healing activity.
从费氏江蓠中分离出的硫酸化半乳聚糖（SG）被部分降解（DSG），随后分别补充了辛酰基（DSGO）和硫酸根基团（DSGS）。DSG、DSGO和DSGS的分子量分别为7.87、152.79和97.07 kDa。通过傅里叶变换红外光谱（FTIR）和核磁共振（NMR）对修饰进行了确认，同时使用划痕伤口成纤维细胞评估体外伤口愈合活性。结果显示，DSGO在划痕的成纤维细胞L929中表现出最高的伤口闭合百分比。此外，DSGO能够促进划痕成纤维细胞的增殖并加速其迁移，这与通过蛋白质印迹法和定量聚合酶链反应（qPCR）分析测定的蛋白质和信使核糖核酸（Ki67、磷酸化粘着斑激酶、波形蛋白和E-钙粘蛋白）的调节相对应。DSGO在大鼠切除伤口模型中也证实了其卓越的伤口愈合活性。结果表明，DSGO显著提高了伤口闭合百分比、再上皮化和胶原蛋白排列，增加了α-平滑肌肌动蛋白（α-SMA）和波形蛋白的表达，并降低了伤口部位肿瘤坏死因子-α（TNF-α）的表达。结果表明，补充了辛酰基中链脂肪酸的降解SG可能穿过细胞膜，并随后激活与成纤维细胞增殖和迁移相关的介质，这可能会促进伤口愈合活性。
