Department of Anatomy, Faculty of Science, Mahidol University, Bangkok 10400, Thailand.
Department of Anatomy, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand.
Mar Drugs. 2022 Jul 23;20(8):469. doi: 10.3390/md20080469.
Various seaweed sulfated polysaccharides have been explored for antimicrobial application. This study aimed to evaluate the antibacterial activity of the native sulfated galactans (NSG) and depolymerized fractions against the marine pathogenic bacteria and . NSG was hydrolyzed in different concentrations of HO to generate sulfated galactans degraded fractions (SGF). The molecular weight, structural characteristics, and physicochemical parameters of both NSG and SGF were determined. The results revealed that the high molecular weight NSG (228.33 kDa) was significantly degraded to SGFs of 115.76, 3.79, and 3.19 kDa by hydrolysis with 0.4, 2, and 10% HO, respectively. The Fourier transformed spectroscopy (FTIR) and H- and C-Nuclear magnetic resonance (NMR) analyses demonstrated that the polysaccharide chain structure of SGFs was not affected by HO degradation, but alterations were detected at the peak positions of some functional groups. In vitro study showed that SGFs significantly exerted a stronger antibacterial activity against and than NSG, which might be due to the low molecular weight and higher sulfation properties of SGF. SGF disrupted the bacterial cell membrane, resulting in leakage of intracellular biological components, and subsequently, cell death. Taken together, this study provides a basis for the exploitation and utilization of low-molecular-weight sulfated galactans from to prevent and control the shrimp pathogens.
各种海藻硫酸多糖已被探索用于抗菌应用。本研究旨在评估天然硫酸半乳糖（NSG）和部分降解产物对海洋病原细菌 和 的抗菌活性。NSG 在不同浓度的 HO 中水解，生成硫酸半乳糖降解产物（SGF）。NSG 和 SGF 的分子量、结构特征和物理化学参数均被测定。结果表明，高分子量 NSG（228.33 kDa）经 0.4%、2%和 10%HO 水解后，分别显著降解为分子量为 115.76、3.79 和 3.19 kDa 的 SGF。傅里叶变换光谱（FTIR）和 H-和 C-核磁共振（NMR）分析表明，SGF 的多糖链结构不受 HO 降解的影响，但在一些功能基团的峰位检测到了变化。体外研究表明，SGF 对 和 表现出比 NSG 更强的抗菌活性，这可能是由于 SGF 的低分子量和更高的硫酸化特性。SGF 破坏了细菌细胞膜，导致细胞内生物成分泄漏，随后导致细胞死亡。综上所述，本研究为开发和利用 中的低分子量硫酸半乳糖来预防和控制虾类病原体提供了依据。
