College of Horticulture, Nanjing Agricultural University, 1 Weigang, Nanjing, 210095, China.
Key Laboratory of Horticultural Plant Biology and Germplasm Innovation in East China, Ministry of Agriculture, Nanjing, China.
Plant Cell Rep. 2022 Oct;41(10):1955-1973. doi: 10.1007/s00299-022-02894-7. Epub 2022 Sep 6.
Abscisic acid induced the expression of AsKIN during the recovery period of garlic cryopreservation. AsKIN was identified as a gene involved in cold and osmotic stress resistance. Cryopreservation has been proven to be effective in removing viruses from garlic. However, oxidative damage in cryopreservation has a significant impact on the survival after preservation. Abscisic acid (ABA) has been shown to reduce oxidative stress and promote the survival after cryopreservation. However, it is not clear which genes play important roles in this process. In this study, we added ABA to the dehydration step and analyzed the transcriptomic divergences between the ABA-treated group and the control group in three cryogenic steps (dehydration, unloading and recovery). By short time-series expression miner (STEM) analysis and weighted gene co-expression network analysis (WGCNA), the recovery step was identified as the period of significant changes in gene expression levels in cryopreservation. The addition of ABA promoted the upregulated expression of microtubule-related genes in the recovery step. We further identified AsKIN as a hub gene in the recovery step and verified its function. The results showed that overexpression of AsKIN enhanced the tolerance of Arabidopsis to oxidative stress in cryopreservation, influenced the expression of genes in response to cold and osmotic stress and promoted plant growth after stress. The AsKIN gene is likely to be involved in the plant response to cold stress and osmotic stress. These results reveal the molecular mechanisms of ABA in cryopreservation and elucidate the potential biological functions of the kinesin-14 subfamily.
脱落酸在大蒜冷冻保存的恢复期诱导 AsKIN 的表达。AsKIN 被鉴定为参与冷胁迫和渗透胁迫抗性的基因。冷冻保存已被证明可有效去除大蒜中的病毒。然而，冷冻保存中的氧化损伤对保存后的存活有重大影响。脱落酸（ABA）已被证明可降低氧化应激并促进冷冻保存后的存活。然而，尚不清楚哪些基因在这个过程中发挥重要作用。在这项研究中，我们在脱水步骤中添加了 ABA，并在三个冷冻步骤（脱水、卸载和恢复）中分析了 ABA 处理组和对照组之间的转录组差异。通过短时间序列表达挖掘器（STEM）分析和加权基因共表达网络分析（WGCNA），发现恢复步骤是冷冻保存中基因表达水平发生显著变化的时期。ABA 的添加促进了恢复步骤中小管相关基因的上调表达。我们进一步鉴定 AsKIN 为恢复步骤中的枢纽基因，并验证了其功能。结果表明，AsKIN 的过表达增强了拟南芥在冷冻保存过程中对氧化应激的耐受性，影响了冷胁迫和渗透胁迫响应基因的表达，并促进了胁迫后植物的生长。AsKIN 基因可能参与植物对冷胁迫和渗透胁迫的响应。这些结果揭示了 ABA 在冷冻保存中的分子机制，并阐明了驱动蛋白-14 亚家族的潜在生物学功能。
