[噬菌体T7的DNA依赖性RNA聚合酶的高度选择性亲和标记]
[Highly selective affinity labeling of DNA-dependent RNA-polymerase of bacteriophage T7].
作者信息
Grachev M A, Zaĭchikov E F, Lukhtanov E A, Maksimova T G, Mustaev A A
出版信息
Bioorg Khim. 1987 Apr;13(4):568-70.
PMID:3606678
Abstract
T7 phage RNA polymerase was affinity labelled in the presence of its promoter by treatment with an ATP gamma-derivative (a phosphoamide obtained from 4-(N-chloroethyl, N-methyl)aminobenzylamine, or one of esters obtained from 2-methoxy-4-formylphenol, 4-formylphenol, and 2[N-(4-formylphenyl), N-methyl]-aminoethanol) followed by addition of [alpha-32P]GTP. The most efficient labelling took place with the alkylating phosphoamide reagent.
摘要
在其启动子存在的情况下，通过用ATPγ衍生物（一种由4-（N-氯乙基，N-甲基）氨基苄胺获得的磷酰胺，或由2-甲氧基-4-甲酰基苯酚、4-甲酰基苯酚和2 [N-（4-甲酰基苯基），N-甲基]-氨基乙醇获得的酯之一）处理T7噬菌体RNA聚合酶，随后添加[α-32P]GTP进行亲和标记。使用烷基化磷酰胺试剂时标记效率最高。
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