[用5'-对氟磺酰苯甲酰腺苷对噬菌体T7的依赖DNA的RNA聚合酶进行亲和修饰]
[Affinity modification of DNA-dependent RNA-polymerase of phage T7 with 5'-p-fluorosulfonylbenzoyladenosine].
作者信息
Tunitskaia V L, Mishin A A, Tiurkin V V, Liakhov D L, Rechinskiĭ V O
出版信息
Mol Biol (Mosk). 1988 Nov-Dec;22(6):1642-9.
PMID:3252154
Abstract
The affinity modification of the DNA-dependent RNA-polymerase of bacteriophage T7 was carried out by using the specific irreversible inhibitor, 5'-p-fluorosulfonylbenzoyladenosine. The inhibitor was found to bind to the enzyme's active site; the kinetic constants of the modification were calculated. The stoichiometry of the covalent E.I-complex formed was determined by using the 14C-labeled inhibitor.
摘要
利用特异性不可逆抑制剂5'-对氟磺酰苯甲酰腺苷对噬菌体T7的依赖DNA的RNA聚合酶进行亲和修饰。发现该抑制剂与酶的活性位点结合；计算了修饰的动力学常数。通过使用14C标记的抑制剂确定了所形成的共价E.I-复合物的化学计量。
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