609 个供卵受者周期中 7515 个新鲜和冷冻解冻卵母细胞的同供者周期妊娠结局比较：单中心分析。

Comparison of pregnancy outcomes of 7515 same donor oocyte cycle fresh and cryopreserved-thawed oocytes in 609 donor oocyte recipient cycles: A single institution analysis.

机构信息

University of Health Sciences, Istanbul Zeynep Kamil Maternity and Children's Training and Research Hospital, Department of Obstetrics and Gynecology, Istanbul, Turkey.

Acibadem Fulya Hospital, Department of Obstetrics and Gynecology, IVF and Endometriosis Center, Istanbul, Turkey.

出版信息

Eur J Obstet Gynecol Reprod Biol. 2022 Oct;277:110-115. doi: 10.1016/j.ejogrb.2022.08.015. Epub 2022 Sep 1.

DOI:10.1016/j.ejogrb.2022.08.015

PMID:36067608

Abstract

OBJECTIVE

Oocyte donation (OD) cycles have been used extensively over the last decades due to high success regarding live birth rate (LBR). We evaluated the reproductive outcomes of fresh and vitrified sibling oocytes in terms of fertilization rates, blast ratio, clinical pregnancy rates, and LBR.

MATERIALS AND METHODS

This retrospective cohort study was conducted at a tertiary in vitro fertilization (IVF) center. A total of 7515 metaphase II (MII) donor oocytes from 304 donor cycles for 609 oocyte recipients undergoing embryo transfers with either fresh or cryopreserved-thawed donor oocytes. Donor cycles that provided both 12 fresh MII oocytes to be used for one recipient and at least 12 MII oocytes which were suitable for vitrification to be used for another recipient at another time were analyzed. Fertilization rates, blastocyst ratios, clinical pregnancy rates (CPR), LBR were evaluated as main outcome measures.

RESULTS

When the fresh and cryopreserved-thawed OD cycles were compared, there was no significant differences between two groups in terms of age of the recipient (41.9 ± 5.7 and 40.3 ± 6.8, p = NS), number of MII oocytes (12.1 ± 0.3 and 12.6 ± 0.8, p = NS), number of 2 pronuclear (PN) (9.1 ± 1.6 and 9.7 ± 2.0, p = NS), blastocyst ratio (58.9 ± 21.7 and 51.3 ± 21.2, p = NS) and number of transferred embryos (1.9 ± 0.3 and 1.9 ± 0.3, p = NS). There was no significant difference between fresh and cryopreserved-thawed sibling donor oocyte cycles in terms of CPR (66.8 % and 60.7 % respectively, p = NS) or LBR (59.5 % and 55.1 %, respectively; p = NS). Miscarriage and multiple gestation rates were similar between groups (p = NS). Log-binomial regression analysis revealed that the use of fresh sibling oocytes was not associated with CPR or LBR, when compared to cryopreserved-thawed oocytes.

CONCLUSIONS

There was no significant difference in terms of reproductive outcomes between sibling fresh OD and cryopreserved-thawed OD cycles.

摘要

目的

由于活产率（LBR）高，卵母细胞捐赠（OD）周期在过去几十年中得到了广泛应用。我们评估了新鲜和冷冻保存的同胞卵母细胞在受精率、囊胚比、临床妊娠率和 LBR 方面的生殖结局。

材料和方法

这是一项在一家三级体外受精（IVF）中心进行的回顾性队列研究。共有 609 名接受胚胎移植的受体接受了 304 个供体周期的 7515 个中期 II（MII）供体卵母细胞，这些卵母细胞来自新鲜或冷冻解冻的供体卵母细胞。分析了同时提供 12 个新鲜 MII 卵母细胞用于一名受体和至少 12 个 MII 卵母细胞适合在另一次用于另一名受体的冷冻保存的供体周期。受精率、囊胚比、临床妊娠率（CPR）和活产率（LBR）被评估为主要观察指标。

结果

当比较新鲜和冷冻解冻的 OD 周期时，两组受体的年龄（41.9±5.7 和 40.3±6.8，p=NS）、MII 卵母细胞数量（12.1±0.3 和 12.6±0.8，p=NS）、2 原核（PN）的数量（9.1±1.6 和 9.7±2.0，p=NS）、囊胚比（58.9±21.7 和 51.3±21.2，p=NS）和转移胚胎数量（1.9±0.3 和 1.9±0.3，p=NS）无显著差异。新鲜和冷冻解冻的同胞供体卵母细胞周期的 CPR（分别为 66.8%和 60.7%，p=NS）或 LBR（分别为 59.5%和 55.1%，p=NS）无显著差异。两组的流产和多胎妊娠率相似（p=NS）。Log-binomial 回归分析显示，与冷冻保存的卵母细胞相比，使用新鲜的同胞卵母细胞与 CPR 或 LBR 无关。