School of Chemistry, Xi'an Jiaotong University, Xi'an, Shaanxi, 710049, PR China.
School of Chemistry, Xi'an Jiaotong University, Xi'an, Shaanxi, 710049, PR China.
Talanta. 2023 Jan 15;252:123895. doi: 10.1016/j.talanta.2022.123895. Epub 2022 Aug 30.
The separation and purification of proteins is an essential precondition for proteomics research because of the intricate matrix environment. Hence, a facile method has been developed to synthesize hyperbranched polyethyleneimine modified magnetic nanomaterials (FeO-NH-BPEI) with dendritic structure, unique electrostatic effect, and abundant functional groups for the selective adsorption of proteins which greatly avoids the drawbacks of time consumption and leakage of metal ions in traditional pre-treatment. The preparation conditions, physical and chemical properties, and adsorption performance of FeO-NH-BPEI have been fully studied. The obtained materials have stable crystal shape, adequate superparamagnetism, fast adsorption kinetics, high adsorption amount, and admirable reusability. In addition, the as-prepared FeO-NH-BPEI exhibits good selective adsorption ability for electronegative proteins in the neutral solution, exhibiting a potential value for special adsorption for proteins with different pI.
蛋白质的分离和纯化是蛋白质组学研究的必要前提，因为其所处的基质环境非常复杂。因此，我们开发了一种简便的方法，合成具有树枝状结构、独特静电效应和丰富官能团的超支化聚乙烯亚胺修饰的磁性纳米材料（FeO-NH-BPEI），用于选择性吸附蛋白质，这极大地避免了传统预处理中耗时长和金属离子泄漏的缺点。我们充分研究了 FeO-NH-BPEI 的制备条件、物理化学性质和吸附性能。所得到的材料具有稳定的晶体形状、足够的超顺磁性、快速的吸附动力学、高的吸附量和良好的可重复使用性。此外，所制备的 FeO-NH-BPEI 在中性溶液中对带负电荷的蛋白质表现出良好的选择性吸附能力，对于不同等电点的蛋白质具有特殊吸附的潜在价值。
