基于葡萄糖的无膜单酶生物燃料电池的研制。

Development of a membraneless single-enzyme biofuel cell powered by glucose.

机构信息

NanoTechnas - Center of Nanotechnology and Materials Science, Institute of Chemistry, Faculty of Chemistry and Geosciences, Vilnius University, Naugarduko St. 24, LT-03225 Vilnius, Lithuania.

出版信息

Biosens Bioelectron. 2022 Nov 15;216:114657. doi: 10.1016/j.bios.2022.114657. Epub 2022 Aug 28.

DOI:10.1016/j.bios.2022.114657

PMID:36067718

Abstract

This work presents the development of a membraneless single-enzyme biofuel cell powered by glucose (GBFC). The GBFC biocathode is based on a graphite rod electrode (GRE) coated with a layer of Prussian blue (PB) nanoparticles entrapped into poly(pyrrole-2-carboxylic acid) (PPCA) shell, an additional layer of PPCA, and covalently to polymer linked glucose oxidase (GOx). The bioanode is based on GRE modified with a nanobiocomposite composed of poly(1,10-phenanthroline-5,6-dione), gold nanoparticles entrapped in a PPCA shell, and GOx linked by an amide bond to polymer. The operation of the developed single-enzyme GBFC is based on GOx-catalysed oxidation of glucose on both the bioanode and biocathode and reduction of HO electrocatalysed by PB on the biocathode. The GBFC operated in O-saturated buffer medium pH 6.0 containing glucose. An open-circuit voltage (OCV) of 646 mV, a maximum power density of 10.94 μW/cm and a current density of 60.52 μA/cm at 40 mM glucose were determined. OCV and current density were directly proportional to glucose concentration in 0.01-10.00 mM and 0.05-124.00 mM concentration ranges, respectively. In addition, GBFC had good operational stability and retained more than 90% of the initial OCV after 36 days.

摘要

这项工作提出了一种无膜单酶葡萄糖生物燃料电池（GBFC）的开发。GBFC 的生物阴极基于涂有普鲁士蓝（PB）纳米粒子的石墨棒电极（GRE），这些纳米粒子被包裹在聚（吡咯-2-羧酸）（PPCA）壳中，还有一层额外的 PPCA，以及通过酰胺键与聚合物连接的葡萄糖氧化酶（GOx）。生物阳极基于经过纳米生物复合材料修饰的 GRE，该复合材料由聚（1,10-邻菲啰啉-5,6-二酮）、包裹在 PPCA 壳中的金纳米粒子和通过酰胺键与聚合物连接的 GOx 组成。所开发的单酶 GBFC 的运行基于 GOx 在生物阳极和生物阴极上催化葡萄糖的氧化，以及 PB 在生物阴极上对 HO 的还原进行电催化。GBFC 在含有葡萄糖的 O 饱和缓冲介质 pH 6.0 中运行。在 40 mM 葡萄糖下，开路电压（OCV）为 646 mV，最大功率密度为 10.94 μW/cm，电流密度为 60.52 μA/cm。OCV 和电流密度分别与 0.01-10.00 mM 和 0.05-124.00 mM 浓度范围内的葡萄糖浓度成正比。此外，GBFC 具有良好的运行稳定性，在 36 天后保留了初始 OCV 的 90%以上。