College of Food Science and Technology, Henan Key Laboratory of Cereal and Oil Food Safety Inspection and Control, Henan University of Technology, Zhengzhou, 450001, PR China.
College of Chemistry and Chemical Engineering, Henan University, Kaifeng, 475004, PR China.
Anal Chim Acta. 2022 Sep 15;1226:340272. doi: 10.1016/j.aca.2022.340272. Epub 2022 Aug 18.
Aflatoxin B1 (AFB) has strong carcinogenicity and toxicity, so it is necessary to develop a highly sensitive detection method. In this paper, a novel nitrogen-doped carbon supported palladium (C-N-Pd) material was synthesized and firstly used as the energy receptor for fluorescent aptasensor. Using C-N-Pd as a novel quenching material and exonuclease Ⅲ (Exo III) as assisted signal amplification, a fluorescent aptasensor for AFB detection was constructed. Compared with the sensor without enzyme, the fluorescence intensity obtained by the sensor with Exo III increased by 74.7%. The fabricated fluorescent aptasensor exhibited a good selectivity toward AFB with a limit of detection (LOD) as low as 9 pg mL. Moreover, the designed aptasensor was successfully utilized to detect AFB in spiked corn, peanut and wine samples, and the LOD is 15 pg mL, 13 pg mL and 18 pg mL, respectively. In addition, the aptasensor was also compared with HPLC method, and the good agreement was found between them.
黄曲霉毒素 B1（AFB）具有很强的致癌性和毒性，因此有必要开发一种高灵敏度的检测方法。本文合成了一种新型氮掺杂碳负载钯（C-N-Pd）材料，并首次将其用作荧光适体传感器的能量受体。使用 C-N-Pd 作为新型猝灭材料和外切酶 Ⅲ（Exo III）作为辅助信号放大，构建了用于检测 AFB 的荧光适体传感器。与没有酶的传感器相比，具有 Exo III 的传感器获得的荧光强度增加了 74.7%。所构建的荧光适体传感器对 AFB 表现出良好的选择性，检测限（LOD）低至 9 pg mL。此外，该设计的适体传感器已成功用于检测玉米、花生和酒样中的 AFB，LOD 分别为 15 pg mL、13 pg mL 和 18 pg mL。此外，还将适体传感器与 HPLC 方法进行了比较，发现它们之间具有良好的一致性。
