School of Grain Science and Technology, Jiangsu University of Science and Technology, Zhenjiang, 212003, Jiangsu, China.
School of Environmental and Chemical Engineering, Jiangsu University of Science and Technology, Zhenjiang, 212003, Jiangsu, China.
Mikrochim Acta. 2024 Jun 14;191(7):397. doi: 10.1007/s00604-024-06482-2.
A fluorescence biosensor for determination of aflatoxin B (AFB) based on polydiacetylene (PDA) liposomes and exonuclease III (EXO III)-assisted recycling amplification was developed. The AFB aptamer partially hybridizes with complementary DNA (cDNA), which is released upon recognition of AFB by the aptamer. Subsequently, the cDNA hybridizes with hairpin H to form double-stranded DNA that undergoes digestion by EXO III, resulting in the cyclic release of cDNA and generation of capture DNA for further reaction. The capture DNA then hybridizes with probe modified on PDA liposomes, leading to aggregation of liposomes and subsequent fluorescence production. This strategy exhibited a limit of detection of 0.18 ng/mL within the linear range 1-100 ng/mL with a determination coefficient > 0.99. The recovery ranged from 92.81 to 106.45%, with relative standard deviations (RSD) between 1.73 and 4.26%, for corn, brown rice, peanut butter, and wheat samples. The stability, accuracy, and specificity of the method demonstrated the applicability for real sample analysis.
基于聚二乙炔（PDA）脂质体和exonuclease III（EXO III）辅助循环扩增的荧光生物传感器，用于测定黄曲霉毒素 B（AFB）。当适体识别 AFB 时，AFB 适体部分与互补 DNA（cDNA）杂交，cDNA 被释放。随后，cDNA 与发夹 H 杂交形成双链 DNA，双链 DNA 被 EXO III 消化，导致 cDNA 的循环释放和捕获 DNA 的生成，以进行进一步反应。然后，捕获 DNA 与 PDA 脂质体上修饰的探针杂交，导致脂质体聚集和随后的荧光产生。该策略在 1-100 ng/mL 的线性范围内，检测限为 0.18 ng/mL，测定系数>0.99。在玉米、糙米、花生酱和小麦样品中，回收率在 92.81%至 106.45%之间，相对标准偏差（RSD）在 1.73%至 4.26%之间。该方法的稳定性、准确性和特异性表明其适用于实际样品分析。
