Laboratory of Bioengineering and Regenerative Medicine, Center for Life Sciences, National Laboratory Astana, Nazarbayev University, Nur-Sultan, Kazakhstan.
Graduate School of Public Health, Al-Farabi Kazakh National University, Almaty, Kazakhstan.
Sci Rep. 2022 Sep 6;12(1):15115. doi: 10.1038/s41598-022-19393-0.
We have investigated the diversity and composition of gut microbiotas isolated from AD (Alzheimer's disease) patients (n = 41) and healthy seniors (n = 43) from Nur-Sultan city (Kazakhstan). The composition of the gut microbiota was characterized by 16S ribosomal RNA sequencing. Our results demonstrated significant differences in bacterial abundance at phylum, class, order, and genus levels in AD patients compared to healthy aged individuals. Relative abundance analysis has revealed increased amount of taxa belonging to Acidobacteriota, Verrucomicrobiota, Planctomycetota and Synergistota phyla in AD patients. Among bacterial genera, microbiotas of AD participants were characterized by a decreased amount of Bifidobacterium, Clostridia bacterium, Castellaniella, Erysipelotrichaceae UCG-003, Roseburia, Tuzzerella, Lactobacillaceae and Monoglobus. Differential abundance analysis determined enriched genera of Christensenellaceae R-7 group, Prevotella, Alloprevotella, Eubacterium coprostanoligenes group, Ruminococcus, Flavobacterium, Ohtaekwangia, Akkermansia, Bacteroides sp. Marseille-P3166 in AD patients, whereas Levilactobacillus, Lactiplantibacillus, Tyzzerella, Eubacterium siraeum group, Monoglobus, Bacteroides, Erysipelotrichaceae UCG-003, Veillonella, Faecalibacterium, Roseburia, Haemophilus were depleted. We have also found correlations between some bacteria taxa and blood serum biochemical parameters. Adiponectin was correlated with Acidimicrobiia, Faecalibacterium, Actinobacteria, Oscillospiraceae, Prevotella and Christensenellaceae R-7. The Christensenellaceae R-7 group and Acidobacteriota were correlated with total bilirubin, while Firmicutes, Acidobacteriales bacterium, Castellaniella alcaligenes, Lachnospiraceae, Christensenellaceae and Klebsiella pneumoniae were correlated with the level of CRP in the blood of AD patients. In addition, we report the correlations found between disease severity and certain fecal bacteria. This is the first reported study demonstrating gut microbiota alterations in AD in the Central Asian region.
我们调查了来自哈萨克斯坦努尔苏丹市的 AD（阿尔茨海默病）患者（n=41）和健康老年人（n=43）的肠道微生物组的多样性和组成。通过 16S 核糖体 RNA 测序来描述肠道微生物组的组成。与健康老年人相比，AD 患者在门、纲、目和属水平的细菌丰度存在显著差异。相对丰度分析显示，AD 患者体内属于 Acidobacteriota、Verrucomicrobiota、Planctomycetota 和 Synergistota 门的细菌数量增加。在细菌属中，AD 参与者的微生物组的双歧杆菌、梭状芽胞杆菌、 Castellaniella、Erysipelotrichaceae UCG-003、Roseburia、Tuzzerella、乳杆菌科和 Monoglobus 的数量减少。差异丰度分析确定了 AD 患者中 Christensenellaceae R-7 组、Prevotella、Alloprevotella、Eubacterium coprostanoligenes 组、Ruminococcus、Flavobacterium、Ohtaekwangia、Akkermansia、Bacteroides sp. Marseille-P3166 属的富集，而 Levilactobacillus、Lactiplantibacillus、Tyzzerella、Eubacterium siraeum 组、Monoglobus、Bacteroides、Erysipelotrichaceae UCG-003、Veillonella、Faecalibacterium、Roseburia 和 Haemophilus 属的丰度降低。我们还发现了一些细菌类群与血液生化参数之间的相关性。脂联素与 Acidimicrobiia、Faecalibacterium、Actinobacteria、Oscillospiraceae、Prevotella 和 Christensenellaceae R-7 相关。Christensenellaceae R-7 组和 Acidobacteriota 与总胆红素相关，而 Firmicutes、Acidobacteriales bacterium、Castellaniella alcaligenes、Lachnospiraceae、Christensenellaceae 和 Klebsiella pneumoniae 与 AD 患者血液中的 CRP 水平相关。此外，我们还报告了在疾病严重程度与某些粪便细菌之间发现的相关性。这是首次在中亚地区报道 AD 肠道微生物组改变的研究。
