International Research Center for Marine Biosciences at Shanghai Ocean University, Ministry of Science and Technology, Shanghai, 201306, China.
Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai, 201306, China.
Microb Ecol. 2023 Aug;86(2):1200-1212. doi: 10.1007/s00248-022-02106-5. Epub 2022 Sep 7.
Intestinal microbes are essential participants in host vital activities. The composition of the microbiota is closely related to the environmental factors. Predator presence may impact on intestinal microbiota of prey. In the present study, stone crab Charybdis japonica was used as potential predator, an external stress on mussel Mytilus coruscus, to investigate the intestinal microbiota alteration in M. coruscus. We set up two forms of predator presence including free crab and trapped crab, with a blank treatment without crab. The composition of intestinal microbiota in mussels among different treatments showed significant differences by 16S rRNA techniques. The biodiversity increased with trapped crab presence, but decreased with free crab presence. Neisseria, the most abundant genus, fell with the presence of crabs. Besides, the Arcobacter, a kind of pathogenic bacteria, increased with free crab presence. Regarding PICRUTs analysis, Environmental Information Processing, Genetic Information Processing and Metabolism showed differences in crab presence treatments compared with the blank, with a bit higher in the presence of free crab than trapped crab. In conclusion, trapped crab effects activated the metabolism and immunity of the intestinal flora, but free crabs made mussels more susceptible to disease and mortality, corresponding to the decreased biodiversity and the increased Arcobacter in their intestine.
肠道微生物是宿主生命活动的重要参与者。微生物群落的组成与环境因素密切相关。捕食者的存在可能会影响猎物的肠道微生物群。本研究以石蟹 Charybdis japonica 为潜在捕食者，以贻贝 Mytilus coruscus 为外部胁迫，研究 M. coruscus 肠道微生物群的变化。我们设置了两种形式的捕食者存在方式，包括自由蟹和被困蟹，以及没有蟹的空白处理。通过 16S rRNA 技术，不同处理组贻贝肠道微生物群落的组成存在显著差异。在被困蟹存在的情况下，生物多样性增加，但在自由蟹存在的情况下则减少。丰度最高的属是奈瑟菌，随着蟹的存在而减少。此外，弧菌属，一种致病菌，随着自由蟹的存在而增加。关于 PICRUTs 分析，与空白相比，在有蟹存在的处理中，环境信息处理、遗传信息处理和代谢都存在差异，自由蟹存在的处理比被困蟹存在的处理略高。总之，被困蟹的存在激活了肠道菌群的代谢和免疫，但自由蟹使贻贝更容易患病和死亡，这与它们肠道中生物多样性的减少和弧菌属的增加相对应。
