Department of Biology, Faculty of Science, Istanbul University, Vezneciler, 34134, Istanbul, Turkey.
Department of Biology, Faculty of Science, Ege University, Bornova, 35040, Izmir, Turkey.
Environ Monit Assess. 2020 Feb 19;192(3):186. doi: 10.1007/s10661-020-8129-1.
Mytilus galloprovincialis is a marine mollusk belonging to the Bivalvia class. It has been distributed largely in Turkish shores and worldwide aquatic environments. Besides being known as an environmental pollution indicator, it is highly consumed as a food and has a high economic value. Due to their nutritional mechanisms by filtering water, they are affected by pollution in seawater and mussels can host-microbial diversity of environmental origin as well as pathogenic bacteria. Therefore, in this study, bacterial species found in Mediterranean mussels collected from the coastal stations of Istanbul [Rumeli Kavagi (RK), Kucukcekmece (KC)], and Izmir [(Foca (MF), Urla (MU)] were investigated and compared with microbiological and metagenomic analyses. According to microbiological analysis results, 34 mussel-associated Enterobacteriaceae and Vibrionaceae family members were identified. As a result of the culture-independent metagenomic analysis, taxonomic groups for each station were identified and compared based on Operational Taxonomic Unit data. For all stations, the most abundant bacterial genera were the unclassified bacterial genera. The total number of mussel-related total richness identified in all groups was 4889 (RK = 1605; KC = 1930; MF = 1508; and MU = 1125). According to the metagenomic data obtained in this study, different relative amounts of Lachnospiraceae and Bacteroidetes taxa groups were reported for all stations. The pathogenic bacterial genera identified by metagenomic analyses which may be significant for the public health are Arcobacter, Clostridium, Aeromonas, Vibrio, Escherichia_Shigella, Klebsiella, Campylobacter, Helicobacter, Pseudomonas, Morganella, Serratia, Corynebacterium, Enterococcus, Staphylococcus, Yersinia, Mycoplasma, Brucellaceae_unclassified, Pantoea, and Proteus.
厚壳贻贝是一种属于双壳类的海洋软体动物。它广泛分布于土耳其海岸和世界水生环境中。除了作为环境污染的指标外，它还被大量食用，具有很高的经济价值。由于它们通过过滤水来获取营养的机制，它们会受到海水中污染的影响，并且贻贝可以容纳环境来源的微生物多样性以及致病菌。因此，在这项研究中，对从伊斯坦布尔沿海站（鲁梅利卡瓦吉[RK]、小切克梅切[KC]）和伊兹密尔[福卡[MF]、乌尔拉[MU]）采集的地中海贻贝中发现的细菌种类进行了调查，并与微生物和宏基因组分析进行了比较。根据微生物分析结果，鉴定出 34 种与贻贝相关的肠杆菌科和弧菌科家族成员。由于非培养宏基因组分析，根据操作分类单元数据对每个站点的分类群进行了鉴定和比较。对于所有站点，最丰富的细菌属是未分类的细菌属。在所有组中鉴定的与贻贝相关的总丰富度的总数为 4889（RK=1605；KC=1930；MF=1508；MU=1125）。根据本研究获得的宏基因组数据，所有站点报告了不同相对丰度的 Lachnospiraceae 和拟杆菌门分类群。通过宏基因组分析鉴定的可能对公共健康有重大影响的致病细菌属是弧菌属、梭菌属、气单胞菌属、弧菌属、大肠杆菌属/志贺氏菌属、克雷伯菌属、弯曲菌属、幽门螺杆菌属、假单胞菌属、摩根菌属、沙雷氏菌属、棒状杆菌属、肠球菌属、葡萄球菌属、耶尔森氏菌属、支原体属、布鲁氏菌科未分类、肠杆菌属、普雷沃氏菌属。
