使用患者特异性 hiPSC 衍生心肌细胞高通量钙成像进行候选药物的药物基因组学筛选。
Pharmacogenomic Screening of Drug Candidates using Patient-Specific hiPSC-Derived Cardiomyocyte High-Throughput Calcium Imaging.
机构信息
Department of Pharmacology, Northwestern University Feinberg School of Medicine, Chicago, IL, USA.
Center for Pharmacogenomics, Northwestern University Feinberg School of Medicine, Chicago, IL, USA.
出版信息
Methods Mol Biol. 2022;2547:241-253. doi: 10.1007/978-1-0716-2573-6_10.
Calcium imaging is an invaluable technique to detect and characterize calcium flux in cells. The use of calcium dye provides information on the concentration and spatial distribution of calcium. Calcium imaging is a well-established technique to assess the calcium-induced calcium release mechanism in cardiomyocytes. It can also be used to characterize mutations in genes crucial for this mechanism that frequently causes arrhythmia. Here we describe a high-throughput methodology of calcium imaging that records individual calcium transients in more than 10,000 human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) in less than 30 min.
钙成像技术是一种非常有价值的技术，可以检测和描述细胞内钙离子的流动。钙染料的使用提供了关于钙离子浓度和空间分布的信息。钙成像技术是评估心肌细胞中钙诱导钙释放机制的一种成熟技术。它还可以用于描述导致心律失常的关键基因的突变。在这里，我们描述了一种高通量的钙成像方法，该方法可以在不到 30 分钟的时间内记录超过 10000 个人诱导多能干细胞衍生的心肌细胞（hiPSC-CMs）中的单个钙瞬变。
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