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Pharmacogenomic Screening of Drug Candidates using Patient-Specific hiPSC-Derived Cardiomyocyte High-Throughput Calcium Imaging.使用患者特异性 hiPSC 衍生心肌细胞高通量钙成像进行候选药物的药物基因组学筛选。
Methods Mol Biol. 2022;2547:241-253. doi: 10.1007/978-1-0716-2573-6_10.
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Human-induced pluripotent stem cell-derived cardiomyocytes from cardiac progenitor cells: effects of selective ion channel blockade.源自心脏祖细胞的人诱导多能干细胞衍生心肌细胞：选择性离子通道阻断的作用
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Independent compartmentalization of functional, metabolic, and transcriptional maturation of hiPSC-derived cardiomyocytes.人诱导多能干细胞衍生心肌细胞功能、代谢和转录成熟的独立区隔化。
Cell Rep. 2024 May 28;43(5):114160. doi: 10.1016/j.celrep.2024.114160. Epub 2024 Apr 27.
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Personalized medicine in the dish to prevent calcium leak associated with short-coupled polymorphic ventricular tachycardia in patient-derived cardiomyocytes.个体化医学培养皿防止钙漏与患者来源的心肌细胞中短联多变型室性心动过速相关。
Stem Cell Res Ther. 2023 Sep 23;14(1):266. doi: 10.1186/s13287-023-03502-5.
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Merits of hiPSC-Derived Cardiomyocytes for In Vitro Research and Testing Drug Toxicity.人诱导多能干细胞衍生心肌细胞用于体外研究和药物毒性测试的优点。
Biomedicines. 2022 Oct 31;10(11):2764. doi: 10.3390/biomedicines10112764.

本文引用的文献

1
Calcium Signaling and Cardiac Arrhythmias.钙信号与心律失常
Circ Res. 2017 Jun 9;120(12):1969-1993. doi: 10.1161/CIRCRESAHA.117.310083.
2
Twenty years of calcium imaging: cell physiology to dye for.二十年的钙成像：细胞生理学值得为之付出。
Mol Interv. 2005 Apr;5(2):112-27. doi: 10.1124/mi.5.2.8.
3
Cardiac excitation-contraction coupling.心脏兴奋-收缩偶联
Nature. 2002 Jan 10;415(6868):198-205. doi: 10.1038/415198a.
4
Calcium sparks: elementary events underlying excitation-contraction coupling in heart muscle.钙火花：心肌兴奋-收缩偶联的基本事件。
Science. 1993 Oct 29;262(5134):740-4. doi: 10.1126/science.8235594.
5
Calcium transients during excitation-contraction coupling in mammalian heart: aequorin signals of canine Purkinje fibers.哺乳动物心脏兴奋-收缩偶联过程中的钙瞬变：犬浦肯野纤维的水母发光蛋白信号
Science. 1980 Mar 7;207(4435):1085-7. doi: 10.1126/science.7355274.
6
Calcium transients in single muscle fibers.单根肌纤维中的钙瞬变。
Biochem Biophys Res Commun. 1967 Oct 26;29(2):229-34. doi: 10.1016/0006-291x(67)90592-x.

使用患者特异性 hiPSC 衍生心肌细胞高通量钙成像进行候选药物的药物基因组学筛选。

Pharmacogenomic Screening of Drug Candidates using Patient-Specific hiPSC-Derived Cardiomyocyte High-Throughput Calcium Imaging.

机构信息

Department of Pharmacology, Northwestern University Feinberg School of Medicine, Chicago, IL, USA.

Center for Pharmacogenomics, Northwestern University Feinberg School of Medicine, Chicago, IL, USA.

出版信息

Methods Mol Biol. 2022;2547:241-253. doi: 10.1007/978-1-0716-2573-6_10.

DOI:10.1007/978-1-0716-2573-6_10
PMID:36068467
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11247644/
Abstract

Calcium imaging is an invaluable technique to detect and characterize calcium flux in cells. The use of calcium dye provides information on the concentration and spatial distribution of calcium. Calcium imaging is a well-established technique to assess the calcium-induced calcium release mechanism in cardiomyocytes. It can also be used to characterize mutations in genes crucial for this mechanism that frequently causes arrhythmia. Here we describe a high-throughput methodology of calcium imaging that records individual calcium transients in more than 10,000 human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) in less than 30 min.

摘要

钙成像技术是一种非常有价值的技术，可以检测和描述细胞内钙离子的流动。钙染料的使用提供了关于钙离子浓度和空间分布的信息。钙成像技术是评估心肌细胞中钙诱导钙释放机制的一种成熟技术。它还可以用于描述导致心律失常的关键基因的突变。在这里，我们描述了一种高通量的钙成像方法，该方法可以在不到 30 分钟的时间内记录超过 10000 个人诱导多能干细胞衍生的心肌细胞（hiPSC-CMs）中的单个钙瞬变。