State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academic of Sciences, Wuhan 430072, China.
State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academic of Sciences, Wuhan 430072, China; University of Chinese Academy of Sciences, Beijing 100049, China.
Ecotoxicol Environ Saf. 2022 Aug;241:113832. doi: 10.1016/j.ecoenv.2022.113832. Epub 2022 Jul 4.
Rapid development of aquaculture industry and increasing demand of various inputs (especially antibiotics), are suspected to promote the occurrence and spread of ARGs in aquaculture related environments. However, the occurrences of ARGs under different freshwater aquaculture practices are rarely known. Here, we investigated the seasonal profiles of the main ARGs, intI1 and bacteria in waters from three kinds of predominant freshwater aquaculture practices around the Honghu Lake (China), as well as their co-occurrences and interrelationships with antibiotics, heavy metals and general water quality. The results indicate that quinolone resistance genes (qnrB), tetracycline resistance genes (tetB and tetX) and sulfonamide resistance genes (sul1 and sul2) were the top five predominant ARGs with seasonal variations of abundance. Fish ponds were of the highest absolute abundances of tested ARGs than the other two modes. Crayfish ponds and their adjacent ditches shared similar ARGs profile. Different subtypes of ARGs belonging to the same class of resistance were varied in abundances. Some bacteria were predicted to carry different ARGs, which indicating multi-antibiotic resistances. Moreover, the combined environmental factors (antibiotics, heavy metals and water quality) partially shaped the profiles of ARGs and bacteria composition. Overall, this study provides new comprehensive understanding on the characterization of ARGs contamination in different freshwater aquaculture practices from the perspectives of environmental chemistry, microbiology and ecology. The results would benefit the optimization of aquaculture practices toward environmental integrity and sustainability.
水产养殖业的快速发展和各种投入品（尤其是抗生素）需求的增加，被怀疑是促进水产养殖相关环境中 ARGs 的发生和传播的原因。然而，不同淡水养殖方式下 ARGs 的发生情况却鲜为人知。在这里，我们调查了三种主要淡水养殖方式（中国洪湖周边）水体中主要 ARGs（intI1 和细菌）的季节性分布情况，以及它们与抗生素、重金属和一般水质的共存和相互关系。结果表明，喹诺酮类耐药基因（qnrB）、四环素类耐药基因（tetB 和 tetX）和磺胺类耐药基因（sul1 和 sul2）是丰度存在季节性变化的前 5 种主要 ARGs。与其他两种模式相比，鱼塘的测试 ARGs 绝对丰度最高。小龙虾池塘及其相邻沟渠具有相似的 ARGs 分布。属于同一耐药类别的不同亚型的 ARGs 丰度不同。一些细菌被预测携带不同的 ARGs，这表明它们具有多抗生素耐药性。此外，组合环境因素（抗生素、重金属和水质）部分塑造了 ARGs 和细菌组成的分布。总的来说，本研究从环境化学、微生物学和生态学的角度，提供了对不同淡水养殖方式中 ARGs 污染特征的新的全面认识。研究结果将有助于优化水产养殖实践，实现环境完整性和可持续性。
