König Simon, Rzeppa Sebastian, Thieme Detlef, Keiler Annekathrin M
Institute of Doping Analysis and Sports Biochemistry Dresden, Kreischa, Germany.
Environmental Monitoring & Endocrinology, Faculty of Biology, Technische Universität Dresden, Dresden, Germany.
Drug Test Anal. 2024 Aug;16(8):761-765. doi: 10.1002/dta.3365. Epub 2022 Sep 8.
The steroid module of the Athlete Biological Passport (ABP) is based on the analysis of six endogenous steroids in urine samples and a Bayesian statistical approach. However, the urinary steroid concentrations may be affected by confounders like microbial degradation, possible co-administration of diuretics as masking agents, insufficient conjugate hydrolysis or UGT2B17 gene polymorphisms affecting glucuronidation. Therefore, it can be helpful to use other matrices (ABP blood and serum samples) to quantify steroids and thereby support noticeable deviations in the Athlete Biological Passport, for example, abnormally increased urinary testosterone/epitestosterone (T/E) ratios. Aim of the study was to investigate the feasibility to re-use plasma obtained from athlete ABP blood samples for measuring a steroid profile. Therefore, testosterone, androstenedione, cortisol and cortisone were quantified in 36 intra-individual matching ABP blood and serum samples. The steroid levels measured in both matrices showed a high agreement indicating a good stability uninfluenced by storage temperature and duration. Our results pointed out the possibility to expand the athlete ABP blood analysis for steroid profiling.
运动员生物护照（ABP）的类固醇模块基于对尿液样本中六种内源性类固醇的分析以及贝叶斯统计方法。然而，尿类固醇浓度可能会受到微生物降解、可能同时使用利尿剂作为掩盖剂、共轭水解不足或影响葡萄糖醛酸化的UGT2B17基因多态性等混杂因素的影响。因此，使用其他基质（ABP血液和血清样本）来定量类固醇，从而支持运动员生物护照中明显的偏差，例如尿睾酮/表睾酮（T/E）比值异常升高，可能会有所帮助。本研究的目的是探讨重新利用从运动员ABP血液样本中获得的血浆来测量类固醇谱的可行性。因此，对36份个体内匹配的ABP血液和血清样本中的睾酮、雄烯二酮、皮质醇和可的松进行了定量。在两种基质中测得的类固醇水平显示出高度一致性，表明其稳定性良好，不受储存温度和时间的影响。我们的结果指出了扩大运动员ABP血液类固醇谱分析的可能性。
