College of Chemical Engineering and Materials Science, Quanzhou Normal University, Quanzhou 362000, Fujian Province, P. R. China.
College of Chemistry and Materials Science, Shanghai Normal University, Shanghai 200234, China.
J Mater Chem B. 2022 Oct 5;10(38):7789-7796. doi: 10.1039/d2tb01076c.
The rapid and reliable determination of thrombomodulin (TM) is of great significance for the diagnosis of disseminated intravascular coagulation, thrombosis and others. This work exhibits an electrochemiluminescent (ECL) sensor, which was prepared using Ru(bpy) encapsulated by MIL-NH-101(Al) nanocomposites for the sensitive detection of the new-thrombus marker thrombomodulin (TM) for the first time. Specifically, on one hand, with the advantages of high specific surface area, large hollow porous structure and favorable biocompatibility, MIL-NH-101(Al) could load a large amount of luminescent Ru(bpy) and thereby greatly enhance the ECL signal of the immunosensor. On the other hand, KSO is used as co-reactant to form a reduction-oxidation ECL system for cathodic ECL detection with strong anti-interference capacity. The experimental results show that the ECL signal intensity of the Ru(bpy)@MIL-NH-101(Al)-based immunosensor decreased with the immunocapturing of TM, exhibiting a linear detection concentration ranging from 1 × 10 to 10 μg mL and the limit of detection (LOD) of 8.2 × 10 μg mL (S/N = 3). With its ideal stability, selectivity and reproducibility, the proposed ECL immunosensor can provide excellent aid and shows great promise for the detection of TM.
快速可靠地测定血栓调节蛋白（TM）对于弥散性血管内凝血、血栓形成等疾病的诊断具有重要意义。本工作首次展示了一种基于 Ru(bpy) 封装的 MIL-NH-101(Al) 纳米复合材料的电化学发光（ECL）传感器，用于灵敏检测新血栓标志物血栓调节蛋白（TM）。具体而言，一方面，MIL-NH-101(Al) 具有高比表面积、大的中空多孔结构和良好的生物相容性等优点，可负载大量的发光 Ru(bpy)，从而大大增强了免疫传感器的 ECL 信号。另一方面，KSO 用作共反应物，形成还原-氧化 ECL 体系，用于具有强抗干扰能力的阴极 ECL 检测。实验结果表明，基于 Ru(bpy)@MIL-NH-101(Al) 的免疫传感器的 ECL 信号强度随 TM 的免疫捕获而降低，表现出线性检测浓度范围为 1×10 到 10μg mL ，检测限（LOD）为 8.2×10μg mL（S/N=3）。该 ECL 免疫传感器具有理想的稳定性、选择性和重现性，可为 TM 的检测提供出色的辅助，并显示出巨大的应用前景。
