Key Laboratory of Interfacial Reaction & Sensing Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan, 250022, PR China.
Key Laboratory of Interfacial Reaction & Sensing Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan, 250022, PR China.
Biosens Bioelectron. 2019 Oct 1;142:111517. doi: 10.1016/j.bios.2019.111517. Epub 2019 Jul 16.
An electrochemiluminescence (ECL) analytical platform was proposed for ultrasensitive detection of amyloid-β proteins (Aβ) based on the ECL resonance energy transfer (ECL-RET). In this work, gold nanoparticles-functionalized graphitic carbon nitride nanosheets (g-CN@Au NPs) and palladium nanoparticles-coated Metal organic framework (Pd NPs@NH-MIL-53) were synthesized, which were as ECL donor and ECL acceptor respectively. A strong cathode ECL emission was obtained from the g-CN@Au NPs when used KSO as its co-reactant. Here, Au NPs not only was used as an accelerator to enhance and stabilize the ECL signal, but also a connector for attaching Aβ antibody. In addition, NH-MIL-53(Al) was selected as a label material for supporting Pd NPs to synergistically increase the intensity and range of UV-visible absorption. The ECL signal of g-CN@Au NPs was intensely decreased when the ECL acceptor probe Pd NPs@NH-MIL-53 was incubated onto the modified GCE by way of the specific recognition. Under the optimal condition, a wide detection range from 10 fg/mL to 50 ng/mL and a low detection limit of 3.4 fg/mL (S/N = 3) were obtained. In consideration of favorable specificity, stability and reproducibility, the proposed method was successfully applied for Aβ detection in actual human serum samples and could be a potential analytical tool for sensitive molecular trace detection in clinical analysis.
基于电致化学发光共振能量转移（ECL-RET），提出了一种用于检测淀粉样β蛋白（Aβ）的电化学发光（ECL）分析平台。在这项工作中，合成了金纳米粒子功能化石墨相氮化碳纳米片（g-CN@Au NPs）和钯纳米粒子包覆的金属有机骨架（Pd NPs@NH-MIL-53），分别作为 ECL 供体和 ECL 受体。当使用 KSO 作为共反应物时，g-CN@Au NPs 获得了强阴极 ECL 发射。在这里，Au NPs 不仅用作增强和稳定 ECL 信号的加速剂，还用作附着 Aβ抗体的连接器。此外，NH-MIL-53（Al）被选为支持 Pd NPs 的标记材料，以协同增加强度和范围的紫外可见吸收。当 ECL 受体探针 Pd NPs@NH-MIL-53 通过特异性识别被孵育到修饰的 GCE 上时，g-CN@Au NPs 的 ECL 信号强烈降低。在最佳条件下，获得了从 10 fg/mL 到 50 ng/mL 的宽检测范围和 3.4 fg/mL（S/N = 3）的低检测限。考虑到良好的特异性、稳定性和重现性，该方法成功应用于实际人血清样品中的 Aβ检测，可作为临床分析中灵敏分子痕量检测的潜在分析工具。
